Preparation and Characterization of PEG-albumin-curcumin Nanoparticles Intended to Treat Breast Cancer
العنوان: | Preparation and Characterization of PEG-albumin-curcumin Nanoparticles Intended to Treat Breast Cancer |
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المؤلفون: | Satheesh Jogala, Arshiya Aafreen, R Thadakapally, Jithan Aukunuru, Mohammed Habibuddin |
المصدر: | Indian Journal of Pharmaceutical Sciences |
بيانات النشر: | Medknow Publications & Media Pvt Ltd, 2016. |
سنة النشر: | 2016 |
مصطلحات موضوعية: | Curcumin, Serum albumin, Pharmaceutical Science, Nanoparticle, 02 engineering and technology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, breast cancer, In vivo, PEG ratio, Organic chemistry, albumin, Chromatography, biology, Chemistry, Albumin, PEGylation, 021001 nanoscience & nanotechnology, Controlled release, long circulating polymeric nanoparticles, Paclitaxel, 030220 oncology & carcinogenesis, desolvation, biology.protein, 0210 nano-technology, Research Paper |
الوصف: | The aim of present research was to prepare novel serum stable long circulating polymeric nanoparticles for curcumin with a modification to the well known and novel nanoparticle albumin bound technology. polyethylene glycol-albumin-curcumin nanoparticles were prepared using serum albumin and poly ethylene glycol using desolvation technique. Nanoparticles were characterized for encapsulation efficiency, particle size and surface morphology. Drug excipient compatibility was determined using fourier transform infrared spectroscopy. Physical state of the drug in the formulations was known by differential scanning colorimetry. In vitro release and solubility of the drug from nanoparticles were determined. In vivo Drug release, tissue uptake and kupffer cell uptake was determined with optimized nanoformulation in rats after intravenous administration. Cell viability assay was determined using breast cancer cell line MD-MB-231. Entrapment efficiency for prepared nanoparticle was above 95%. The polyethylene glycol-albumin-curcumin nanoparticles exhibited an interesting release profile with small initial burst followed by slower and controlled release. Solubility of the drug from the formulation was increased. A sustained release of drug from nanoparticles was observed for 35 days in both in vitro and in vivo studies with the optimized formulation. Polyethylene glycol-albumin-curcumin nanoparticles showed lesser liver and kupffer cell uptake as compared to that of curcumin-albumin nanoparticles suggesting the bestowment of stealthness to nanoparticles with pegylation. Also, the antiproliferative activity of polyethylene glycol-albumin-curcumin nanoparticle formulation was more as compared to native curcumin. Polyethylene glycol-albumin-curcumin nanoparticles thus developed can be conveniently used in breast cancer with improved efficacy compared to conventional therapies and as an alternate to nanoparticle albumin bound technology which is used in producing Abraxane, albumin based breast cancer targeting nanoparticles of paclitaxel. |
اللغة: | English |
تدمد: | 1998-3743 0250-474X |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23e29d44672e40ba9edb84aeacc87f1f http://europepmc.org/articles/PMC4852577 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....23e29d44672e40ba9edb84aeacc87f1f |
قاعدة البيانات: | OpenAIRE |
تدمد: | 19983743 0250474X |
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