Tumor lysate particle loaded dendritic cell vaccine: preclinical testing of a novel personalized cancer vaccine
| العنوان: | Tumor lysate particle loaded dendritic cell vaccine: preclinical testing of a novel personalized cancer vaccine |
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| المؤلفون: | Timothy J. Vreeland, Guy T. Clifton, Doreen O. Jackson, George E. Peoples, Pauline Nichols, Garth S. Herbert, John E Berry, Diane F. Hale, Mark O. Hardin, Julia M. Greene, Sook Yin, Xianzhong Yu, Thomas E. Wagner |
| المصدر: | Immunotherapy. 10:373-382 |
| بيانات النشر: | Future Medicine Ltd, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Lung Neoplasms, Lysis, medicine.medical_treatment, Immunology, Antigen presentation, Melanoma, Experimental, Cancer Vaccines, Mice, 03 medical and health sciences, 0302 clinical medicine, Antigen, Antigens, Neoplasm, In vivo, medicine, Animals, Immunology and Allergy, Chemistry, Dendritic Cells, Immunotherapy, Dendritic cell, Mice, Inbred C57BL, Disease Models, Animal, 030104 developmental biology, Oncology, Cytoplasm, 030220 oncology & carcinogenesis, Cancer research, Female, Cancer vaccine |
| الوصف: | Aim: We developed a novel approach to efficiently deliver autologous tumor antigens to the cytoplasm of dendritic cells (DC) using yeast cell wall particles (YCWP). Materials and Methods: Loading of YCWP, leakage of protein from loaded YCWP and cytoplasmic delivery of YCWP content was assessed using fluorescent-tagged experiments. Spectrophotometric analysis compared the epitope-specific T-cell responses following antigen presentation via YCWP versus exogenous loading. The in vivo effectiveness of tumor lysate (TL) particle loaded DC (TLPLDC) vaccine was assessed using murine melanoma models. Results: In fluorescence-tagged experiments, YCWP efficiently delivered antigen to the cytoplasm of DC. TLPLDC loading was more effective than conventional exogenous loading of DC. Finally, in murine melanoma models, TLPLDC outperformed an analogous dendritoma vaccine. Conclusion: The TLPLDC vaccine is commercially scalable and holds the potential of producing personalized vaccines. |
| تدمد: | 1750-7448 1750-743X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2475524195f10c7af917b5a4fd08a22e https://doi.org/10.2217/imt-2017-0114 |
| رقم الأكسشن: | edsair.doi.dedup.....2475524195f10c7af917b5a4fd08a22e |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 17507448 1750743X |
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