Highly Specific Detection of Five Exotic Quarantine Plant Viruses using RT-PCR
| العنوان: | Highly Specific Detection of Five Exotic Quarantine Plant Viruses using RT-PCR |
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| المؤلفون: | Kook-Hyung Kim, Ji Suk Yu, Jong Seung Lee, Ho Seong Choi, Won Kyong Cho |
| المصدر: | The Plant Pathology Journal, Vol 29, Iss 1, Pp 99-104 (2013) The Plant Pathology Journal |
| بيانات النشر: | Hanrimwon Publishing Company, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | biology, Beet black scorch virus, Rice yellow mottle virus, viruses, fungi, Pelargonium zonate spot virus, Nucleic acid sequence, Beet necrotic yellow vein virus, food and beverages, lcsh:Plant culture, biology.organism_classification, Note, Virology, law.invention, Real-time polymerase chain reaction, law, Plant virus, Quarantine, Eggplant mottled dwarf virus, lcsh:SB1-1110, Agronomy and Crop Science, Gene |
| الوصف: | To detect five plant viruses (Beet black scorch virus, Beet necrotic yellow vein virus, Eggplant mottled dwarf virus, Pelargonium zonate spot virus, and Rice yellow mottle virus) for quarantine purposes, we designed 15 RT-PCR primer sets. Primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. All but one primer set successfully amplified the targets, and gradient PCRs indicated that the optimal temperature for the 14 useful primer sets was 51.9°C. Some primer sets worked well regardless of annealing temperature while others required a very specific annealing temperature. A primer specificity test using plant total RNAs and cDNAs of other plant virus-infected samples demonstrated that the designed primer sets were highly specific and generated reproducible results. The newly developed RT-PCR primer sets would be useful for quarantine inspections aimed at preventing the entry of exotic plant viruses into Korea. |
| اللغة: | English |
| تدمد: | 1598-2254 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::24914116b558fb7fe703bc3d1dca4296 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4174791/ |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....24914116b558fb7fe703bc3d1dca4296 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15982254 |
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