Epigenomic features of DNA G-quadruplexes and their roles in regulating rice gene transcription
| العنوان: | Epigenomic features of DNA G-quadruplexes and their roles in regulating rice gene transcription |
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| المؤلفون: | Yilong Feng, Shentong Tao, Pengyue Zhang, Francesco Rota Sperti, Guanqing Liu, Xuejiao Cheng, Tao Zhang, Hengxiu Yu, Xiu-e Wang, Caiyan Chen, David Monchaud, Wenli Zhang |
| المساهمون: | Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC) |
| المصدر: | Plant Physiology Plant Physiology, American Society of Plant Biologists, In press, ⟨10.1093/plphys/kiab566⟩ Plant Physiol |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Crops, Agricultural, Epigenomics, 2. Zero hunger, 0303 health sciences, Transcription, Genetic, G-quadruplex, Physiology, rice, 030302 biochemistry & molecular biology, epigenomic signatures, food and beverages, Oryza, DNA, Plant Science, Genes, Plant, Plants, Genetically Modified, regulation of gene transcription, G-Quadruplexes, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, 03 medical and health sciences, Gene Expression Regulation, Plant, global mapping, Genetics, Research Articles, 030304 developmental biology |
| الوصف: | A DNA G-quadruplex (G4) is a non-canonical four-stranded nucleic acid structure involved in many biological processes in mammals. The current knowledge on plant DNA G4s, however, is limited; whether and how DNA G4s impact gene expression in plants is still largely unknown. Here, we applied a protocol referred to as BG4-DNA-IP-seq followed by a comprehensive characterization of DNA G4s in rice (Oryza sativa L.); we next integrated dG4s (experimentally detectable G4s) with existing omics data and found that dG4s exhibited differential DNA methylation between transposable element (TE) and non-TE genes. dG4 regions displayed genic-dependent enrichment of epigenomic signatures; finally, we showed that these sites displayed a positive association with expression of DNA G4-containing genes when located at promoters, and a negative association when located in the gene body, suggesting localization-dependent promotional/repressive roles of DNA G4s in regulating gene transcription. This study reveals interrelations between DNA G4s and epigenomic signatures, as well as implicates DNA G4s in modulating gene transcription in rice. Our study provides valuable resources for the functional characterization or bioengineering of some of key DNA G4s in rice. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2588c78c57640eb8ac2796a8f8f1a8aa https://doi.org/10.1093/plphys/kiab566 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2588c78c57640eb8ac2796a8f8f1a8aa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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