Systematic evaluation of PAXgene® tissue fixation for the histopathological and molecular study of lung cancer
| العنوان: | Systematic evaluation of PAXgene® tissue fixation for the histopathological and molecular study of lung cancer |
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| المؤلفون: | Mark Southwood, Tomasz Krenz, Natasha Cant, Manisha Maurya, Jana Gazdova, Perry Maxwell, Claire McGready, Ellen Moseley, Susan Hughes, Peter Stewart, Manuel Salto‐Tellez, Daniel Groelz, Doris Rassl, On behalf of the STRATfix Consortium |
| المصدر: | The Journal of Pathology: Clinical Research Southwood, M, Krenz, T, Cant, N, Maurya, M, Gazdova, J, Maxwell, P, McGready, C, Moseley, E, Hughes, S, Stewart, P, Salto-Tellez, M, Groelz, D & Rassl, D 2020, ' Systematic evaluation of PAXgene® tissue fixation for the histopathological and molecular study of lung cancer ', The Journal of Pathology: Clinical Research, vol. 6, no. 1, pp. 40-54 . https://doi.org/10.1002/cjp2.145, https://doi.org/10.1002/cjp2.145 The Journal of Pathology: Clinical Research, Vol 6, Iss 1, Pp 40-54 (2020) |
| بيانات النشر: | John Wiley & Sons, Inc., 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | medicine.medical_specialty, Pathology, Lung Neoplasms, Tissue Fixation, Histopathology, Polymerase Chain Reaction, DNA sequencing, Pathology and Forensic Medicine, Fixatives, SDG 3 - Good Health and Well-being, Formaldehyde, Parenchyma, lcsh:Pathology, medicine, Biomarkers, Tumor, Humans, Epidermal growth factor receptor, Lung cancer, Fixation (histology), biology, business.industry, Lung Cancer, High-Throughput Nucleotide Sequencing, Original Articles, Sequence Analysis, DNA, medicine.disease, Immunohistochemistry, Staining, biology.protein, Original Article, business, lcsh:RB1-214 |
| الوصف: | Whilst adequate for most existing pathological tests, formalin is generally considered a poor DNA preservative and use of alternative fixatives may prove advantageous for molecular testing of tumour material; an increasingly common approach to identify targetable driver mutations in lung cancer patients. We collected paired PAXgene® tissue‐fixed and formalin‐fixed samples of block‐sized tumour and lung parenchyma, Temno‐needle core tumour biopsies and fine needle tumour aspirates (FNAs) from non‐small cell lung cancer resection specimens. Traditionally processed formalin fixed paraffin wax embedded (FFPE) samples were compared to paired PAXgene® tissue fixed paraffin‐embedded (PFPE) samples. We evaluated suitability for common laboratory tests (H&E staining and immunohistochemistry) and performance for downstream molecular investigations relevant to lung cancer, including RT‐PCR and next generation DNA sequencing (NGS). Adequate and comparable H&E staining was seen in all sample types and nuclear staining was preferable in PAXgene® fixed Temno tumour biopsies and tumour FNA samples. Immunohistochemical staining was broadly comparable. PFPE samples enabled greater yields of less‐fragmented DNA than FFPE comparators. PFPE samples were also superior for PCR and NGS performance, both in terms of quality control metrics and for variant calling. Critically we identified a greater number of genetic variants in the epidermal growth factor receptor gene when using PFPE samples and the Ingenuity® Variant Analysis pipeline. In summary, PFPE samples are adequate for histopathological diagnosis and suitable for the majority of existing laboratory tests. PAXgene® fixation is superior for DNA and RNA integrity, particularly in low‐yield samples and facilitates improved NGS performance, including the detection of actionable lung cancer mutations for precision medicine in lung cancer samples. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 2056-4538 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26062140c9585c9594a011511109a548 http://europepmc.org/articles/PMC6966705 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....26062140c9585c9594a011511109a548 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20564538 |
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