Phenotypes of HeLa S3 variant cell lines resistant to growth inhibition by sodium butyrate
| العنوان: | Phenotypes of HeLa S3 variant cell lines resistant to growth inhibition by sodium butyrate |
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| المؤلفون: | D L. Gendusoday, J. Pensky, A. Milsted, Rody P. Cox |
| المصدر: | In Vitro Cellular & Developmental Biology. 23:395-402 |
| بيانات النشر: | Springer Science and Business Media LLC, 1987. |
| سنة النشر: | 1987 |
| مصطلحات موضوعية: | Cell cycle checkpoint, Cell division, Clinical Biochemistry, Cell, Drug Resistance, Plant Science, Butyrate, Biology, Chorionic Gonadotropin, Histones, chemistry.chemical_compound, Pituitary Hormones, Anterior, medicine, Humans, Chorionic Gonadotropin, beta Subunit, Human, RNA, Messenger, Phosphorylation, Cell growth, Cell Cycle, Genetic Variation, Acetylation, Sodium butyrate, Cell Biology, Cell cycle, Molecular biology, Peptide Fragments, Butyrates, Phenotype, medicine.anatomical_structure, chemistry, Glycoprotein Hormones, alpha Subunit, Cell culture, Butyric Acid, Cell Division, HeLa Cells, Biotechnology, Developmental Biology |
| الوصف: | HeLa cell variants capable of multiplying in the presence of sodium butyrate were used to study the relationship of cell cycle position to human chorionic gonadotropin (hCG) production and regulation of the genes encoding hCG alpha- and beta-subunits. The butyrate-resistant variants exhibit several different stable phenotypes. In wild-type HeLa cells, butyrate arrests cell division and modulates synthesis of alpha- and beta-subunits of glycoprotein hormones by coordinately regulating steady-state levels of their respective mRNAs. Because the variant cell lines replicate, in addition to producing hCG subunits in the presence of butyrate, cell cycle arrest does not seem to be a requirement for expression of glycoprotein hormone genes. Studies of histone modification suggest that neither hyperacetylation of histones H3 and H4 nor dephosphorylation of histones H1 and H2A mediates inhibition of cell replication. In the variants, alpha-subunit and hCG beta levels are independently regulated, as a consequence of independent regulation of alpha- and beta-hCG mRNA levels. Long-term effects of butyrate include derepression of some genes (hCG beta in the variant AO) and repression of others (hCG alpha in variant AO). Moreover, hormone production correlates with the steady-state levels of mRNA for each of the subunits, suggesting that regulation occurs before translation. These findings indicate that the butyrate-resistant variant cell lines are valuable for studies of the molecular mechanisms involved in regulation of expression of ectopic hormones. |
| تدمد: | 1475-2689 0883-8364 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::264964875b21fafd00dd1ab16afb2921 https://doi.org/10.1007/bf02623854 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....264964875b21fafd00dd1ab16afb2921 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14752689 08838364 |
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