Field and saccharification performances of poplars severely downregulated in CAD1
| العنوان: | Field and saccharification performances of poplars severely downregulated in CAD1 |
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| المؤلفون: | Barbara De Meester, Rebecca Van Acker, Marlies Wouters, Silvia Traversari, Marijke Steenackers, Jenny Neukermans, Frank Van Breusegem, Annabelle Déjardin, Gilles Pilate, Wout Boerjan |
| المصدر: | NEW PHYTOLOGIST |
| بيانات النشر: | Wiley, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | water content, Physiology, XYLEM, LIGNIN BIOSYNTHESIS, Biology and Life Sciences, MECHANICAL-PROPERTIES, Plant Science, CINNAMYL-ALCOHOL-DEHYDROGENASE, Lignin, saccharification, Alcohol Oxidoreductases, field trial, Populus, poplar, TRANSGENIC POPLARS, POPULUS-TRICHOCARPA, HYBRID POPLAR, TRADE-OFF, GROWTH, CAD, lignin engineering, PLANTS, Biomass, wood |
| الوصف: | Lignin is one of the main factors causing lignocellulosic biomass recalcitrance to enzymatic hydrolysis. Glasshouse-grown poplars severely downregulated for CINNAMYL ALCOHOL DEHYDROGENASE 1 (CAD1), the enzyme catalysing the last step in the monolignol-specific branch of lignin biosynthesis, have increased saccharification yields and normal growth. Here, we assess the performance of these hpCAD poplars in the field under short rotation coppice culture for two consecutive rotations of 1 yr and 3 yr. While 1-yr-old hpCAD wood had 10% less lignin, 3-yr-old hpCAD wood had wild-type lignin levels. Because of their altered cell wall composition, including elevated levels of cinnamaldehydes, both 1-yr-old and 3-yr-old hpCAD wood showed enhanced saccharification yields upon harsh alkaline pretreatments (up to +85% and +77%, respectively). In contrast with previous field trials with poplars less severely downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD), the hpCAD poplars displayed leaning phenotypes, early bud set, early flowering and yield penalties. Moreover, hpCAD wood had enlarged vessels, decreased wood density and reduced relative and free water contents. Our data show that the phenotypes of CAD-deficient poplars are strongly dependent on the environment and underpin the importance of field trials in translating basic research towards applications. |
| وصف الملف: | application/pdf |
| تدمد: | 1469-8137 0028-646X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2677955afad34251eda083e98821617c https://doi.org/10.1111/nph.18366 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2677955afad34251eda083e98821617c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14698137 0028646X |
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