Maternal nicotinamide supplementation causes global DNA hypomethylation, uracil hypo-incorporation and gene expression changes in fetal rats
| العنوان: | Maternal nicotinamide supplementation causes global DNA hypomethylation, uracil hypo-incorporation and gene expression changes in fetal rats |
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| المؤلفون: | Yan-Jie Tian, Qiang Ma, Ning Luo, Zhi Li, Xin-Yi Gu, Shi-Sheng Zhou, Na-Na Chen, Yong-Zhi Lun |
| المصدر: | British Journal of Nutrition. 111:1594-1601 |
| بيانات النشر: | Cambridge University Press (CUP), 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Niacinamide, medicine.medical_specialty, Offspring, Placenta, Down-Regulation, Medicine (miscellaneous), Biology, DNA methyltransferase, Epigenesis, Genetic, Fetal Development, Rats, Sprague-Dawley, Random Allocation, chemistry.chemical_compound, Fetus, Betaine, Pregnancy, Internal medicine, medicine, Animals, Uracil, Fetal Death, Neurons, Nutrition and Dietetics, Nicotinamide, Brain, Gene Expression Regulation, Developmental, Fetal Body Weight, DNA, Maternal Nutritional Physiological Phenomena, DNA Methylation, Molecular biology, Placentation, Rats, medicine.anatomical_structure, Endocrinology, Liver, chemistry, Dietary Supplements, DNA methylation, Female |
| الوصف: | Recent evidence shows that excess nicotinamide can cause epigenetic changes in developing rats. The aim of the present study was to investigate the effects of maternal nicotinamide supplementation on the fetus. Female rats were randomised into four groups fed a standard chow diet (control group) or diets supplemented with 1 g/kg of nicotinamide (low-dose group), 4 g/kg of nicotinamide (high-dose group) or 4 g/kg of nicotinamide plus 2 g/kg of betaine (betaine group) for 14–16 d before mating and throughout the study. Fetal tissue samples were collected on the 20th day of pregnancy. Compared with the control group, the high-dose group had a higher fetal death rate, and the average fetal body weight was higher in the low-dose group but lower in the high-dose group. Nicotinamide supplementation led to a decrease in placental and fetal hepatic genomic DNA methylation and genomic uracil contents (a factor modifying DNA for diversity) in the placenta and fetal liver and brain, which could be completely or partially prevented by betaine. Moreover, nicotinamide supplementation induced tissue-specific alterations in the mRNA expression of the genes encoding nicotinamideN-methyltransferase, DNA methyltransferase 1, catalase and tumour protein p53 in the placenta and fetal liver. High-dose nicotinamide supplementation increased fetal hepatic α-fetoprotein mRNA level, which was prevented by betaine supplementation. It is concluded that maternal nicotinamide supplementation can induce changes in fetal epigenetic modification and DNA base composition. The present study raises the concern that maternal nicotinamide supplementation may play a role in the development of epigenetic-related diseases in the offspring. |
| تدمد: | 1475-2662 0007-1145 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::267d48cc1e51b2a7731ed9c6fc5558b3 https://doi.org/10.1017/s0007114513004054 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....267d48cc1e51b2a7731ed9c6fc5558b3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752662 00071145 |
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