Patterned Plasmonic Nanoparticle Arrays for Microfluidic and Multiplexed Biological Assays
| العنوان: | Patterned Plasmonic Nanoparticle Arrays for Microfluidic and Multiplexed Biological Assays |
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| المؤلفون: | William Lum, Jie He, Michelle Boegli, Ian Bruzas, Laura B. Sagle |
| المصدر: | Analytical Chemistry. 87:11407-11414 |
| بيانات النشر: | American Chemical Society (ACS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Medical diagnostic, Fabrication, Surface Properties, Chemistry, Microfluidics, Nanoparticle, Nanotechnology, Microfluidic Analytical Techniques, Surface Plasmon Resonance, Multiplexing, Analytical Chemistry, law.invention, law, Nanoparticles, Biological Assay, Particle Size, Photolithography, Surface plasmon resonance, Plasmon |
| الوصف: | For applications ranging from medical diagnostics and drug screening to chemical and biological warfare detection, inexpensive, rapid-readout, portable devices are required. Localized surface plasmon resonance (LSPR) technologies show substantial promise toward meeting these goals, but the generation of portable, multiplexed and/or microfluidic devices incorporating sensitive nanoparticle arrays is only in its infancy. Herein, we have combined photolithography with Hole Mask Colloidal lithography to pattern uniform nanoparticle arrays for both microfluidic and multiplexed devices. The first proof-of-concept study is carried out with 5- and 7-channel microfluidic devices to acquire one-shot binding curves and protein binding kinetic data. The second proof-of-concept study involved the fabrication of a 96-spot plate that can be inserted into a standard plate reader for the multiplexed detection of protein binding. This versatile fabrication technique should prove useful in next generation chips for bioassays and genetic screening. |
| تدمد: | 1520-6882 0003-2700 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2694ddd1e64105c8b61363ddd54beb6d https://doi.org/10.1021/acs.analchem.5b02870 |
| رقم الأكسشن: | edsair.doi.dedup.....2694ddd1e64105c8b61363ddd54beb6d |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15206882 00032700 |
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