Simultaneous detection of three amphenicol antibiotics in shrimp and surface water samples by LC–MS/MS using two-antibodies-immobilized immunoaffinity clean-up technique
| العنوان: | Simultaneous detection of three amphenicol antibiotics in shrimp and surface water samples by LC–MS/MS using two-antibodies-immobilized immunoaffinity clean-up technique |
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| المؤلفون: | Jianguo Li, Enhui Guo, Kang Zhao, Linchuan Zhao, Kang Wu, Wen Huang, Anping Deng |
| المصدر: | Food and Agricultural Immunology, Vol 32, Iss 1, Pp 283-297 (2021) |
| بيانات النشر: | Taylor & Francis Group, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Florfenicol, immunoaffinity clean-up, medicine.drug_class, amphenicol antibiotics, Agriculture (General), Immunology, Antibiotics, Monoclonal antibody, 01 natural sciences, S1-972, chemistry.chemical_compound, lc–ms/ms, 0404 agricultural biotechnology, medicine, Chromatography, biology, Chloramphenicol, 010401 analytical chemistry, 04 agricultural and veterinary sciences, RC581-607, Thiamphenicol, 040401 food science, 0104 chemical sciences, Clean-up, Shrimp, chemistry, monoclonal antibody, biology.protein, simultaneous detection, Antibody, Immunologic diseases. Allergy, Agronomy and Crop Science, Food Science, medicine.drug |
| الوصف: | Amphenicol antibiotics including chloramphenicol (CAP), florfenicol (FF) and thiamphenicol (TAP) have been widely used in aquaculture and veterinary to treat various infections. Because of their high toxicity, it is important to detect these amphenicol antibiotics simultaneously. Using the monoclonal antibody against CAP (mAbCAP) binding CAP only and the mAbFF recognizing both FF and TAP, the two antibodies were covalently coupled to CNBr-activated Sepharose-4B for the simultaneous extraction of CAP, FF and TAP from samples. The bound CAP, FF and TAP were eluted from the Sepharose-4B and measured by LC–MS/MS. The extraction conditions of the two-antibodies-immobilized immunoaffinity clean-up (IAC) for CAP, FF and TAP were optimized and the maximum capacity was investigated. The extraction efficiency of IAC for CAP, FF and TAP was 1.1–2.1 times higher than that of SPE. The LC–MS/MS coupled with IAC was a powerful analytical method for the detection of CAP, FF and TAP simultaneously. |
| اللغة: | English |
| تدمد: | 1465-3443 0954-0105 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26afed9ec72e0ea5bcf21234f58cfd24 https://doaj.org/article/fb1d6db8f81a498b9613c82aa0945e6d |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....26afed9ec72e0ea5bcf21234f58cfd24 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 14653443 09540105 |
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