Vibrio japonicus sp. nov., a novel member of the Nereis clade in the genus Vibrio isolated from the coast of Japan
| العنوان: | Vibrio japonicus sp. nov., a novel member of the Nereis clade in the genus Vibrio isolated from the coast of Japan |
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| المؤلفون: | Manabu Kawada, Ikuko Osawa, Hayamitsu Adachi, Hiroyasu Doi |
| المصدر: | PLoS ONE PLoS ONE, Vol 12, Iss 2, p e0172164 (2017) |
| بيانات النشر: | Public Library of Science, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, lcsh:Medicine, Biochemistry, chemistry.chemical_compound, Database and Informatics Methods, Japan, lcsh:Science, Nereis, Data Management, Base Composition, Multidisciplinary, biology, Strain (chemistry), Ecology, Fatty Acids, Phylogenetic Analysis, Lipids, Anti-Bacterial Agents, Phylogenetics, Sequence Analysis, Research Article, DNA, Bacterial, Computer and Information Sciences, Genotype, Sequence analysis, Bioinformatics, 030106 microbiology, Sequence Databases, Research and Analysis Methods, DNA, Ribosomal, Microbiology, 03 medical and health sciences, Sea Water, Evolutionary Systematics, Seawater, Melibiose, Molecular Biology Techniques, Molecular Biology, DNA sequence analysis, Vibrio, Taxonomy, Molecular Biology Assays and Analysis Techniques, Evolutionary Biology, Bacteria, lcsh:R, Organisms, Biology and Life Sciences, Sequence Analysis, DNA, biology.organism_classification, 16S ribosomal RNA, 030104 developmental biology, Biological Databases, chemistry, Earth Sciences, Multilocus sequence typing, lcsh:Q, Hydrology, Multilocus Sequence Typing |
| الوصف: | A novel Vibrio strain, JCM 31412T, was isolated from seawater collected from the Inland Sea (Setonaikai), Japan, and characterized as a Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, ovoid-shaped bacterium with one polar flagellum. Based on 16S rDNA gene identity, strain JCM 31412T showed a close relationship with type strains of Vibrio brasiliensis (LMG 20546T, 98.2% identity), V. harveyi (NBRC 15634T, 98.2%), V. caribbeanicus (ATCC BAA-2122T, 97.8%) and V. proteolyticus (NBRC 13287T, 97.8%). The G+C content of strain JCM 31412T DNA was 46.8%. Multi-locus sequence analysis (MLSA) of eight loci (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA; 5535bp) further clustered strain JCM 31412T in the Nereis clade, genus Vibrio. Phenotypically, strain JCM 31412T differed from the closest related Vibrio species in its utilization of melibiose and raffinose, and its lack of casein and gelatin hydrolysis. It was further differentiated based on its fatty acid composition, specifically properties of C12:03OH and summed features, which were significantly different from those of V. brasiliensis, V. nigripulchritudo and V. caribbeanicus type strains. Overall, the results of DNA-DNA hybridization, and physiological and biochemical analysis differentiated strain JCM 31412T from other described species of the genus Vibrio. Based on these polyphasic taxonomic findings, it was therefore concluded that JCM 31412T was a novel Vibrio species, for which the name Vibrio japonicus sp. nov. was proposed, with JCM 31412T (= LMG 29636T = ATCC TSD-62T) as the type strain. |
| اللغة: | English |
| تدمد: | 1932-6203 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27182951df4dc673d5d3aa01f2ee4184 http://europepmc.org/articles/PMC5322892 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....27182951df4dc673d5d3aa01f2ee4184 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19326203 |
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