Nano-multilamellar lipid vesicles promote the induction of SARS-CoV-2 immune responses by a protein-based vaccine formulation
| العنوان: | Nano-multilamellar lipid vesicles promote the induction of SARS-CoV-2 immune responses by a protein-based vaccine formulation |
|---|---|
| المؤلفون: | Monica Josiane Rodrigues-Jesus, Marianna Teixeira de Pinho Favaro, Aléxia Adrianne Venceslau-Carvalho, Maria Fernanda de Castro-Amarante, Bianca da Silva Almeida, Mariângela de Oliveira Silva, Robert Andreata-Santos, Cecilia Gomes Barbosa, Samantha Carvalho Maia Brito, Lucio H. Freitas-Junior, Silvia Beatriz Boscardin, Luís Carlos de Souza Ferreira |
| المصدر: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mice, Inbred BALB C, COVID-19 Vaccines, SARS-CoV-2, Biomedical Engineering, Immunity, Pharmaceutical Science, Medicine (miscellaneous), COVID-19, Bioengineering, Antibodies, Viral, Antibodies, Neutralizing, Lipids, Mice, Inbred C57BL, Mice, GLICOPROTEÍNAS, Immunoglobulin G, Spike Glycoprotein, Coronavirus, Vaccines, Subunit, Molecular Medicine, Animals, Humans, General Materials Science |
| الوصف: | The development of safe and effective vaccine formulations against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represents a hallmark in the history of vaccines. Here we report a COVID-19 subunit vaccine based on a SARS-CoV-2 Spike protein receptor binding domain (RBD) incorporated into nano-multilamellar vesicles (NMV) associated with monophosphoryl lipid A (MPLA). The results based on immunization of C57BL/6 mice demonstrated that recombinant antigen incorporation into NMVs improved antibody and T-cell responses without inducing toxic effects under both in vitro and in vivo conditions. Administration of RBD-NMV-MPLA formulations modulated antigen avidity and IgG subclass responses, whereas MPLA incorporation improved the activation of CD4 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27a26619821e81ec29f33e42eeba8645 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....27a26619821e81ec29f33e42eeba8645 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |