Biochemical and Biophysical Characterization of the dsDNA Packaging Motor from the Lactococcus lactis Bacteriophage Asccphi28
| العنوان: | Biochemical and Biophysical Characterization of the dsDNA Packaging Motor from the Lactococcus lactis Bacteriophage Asccphi28 |
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| المؤلفون: | Kyung H. Choi, Priyank Maindola, Michal R. Szymanski, Cecile Bussetta, Mark A. White, Emilio Reyes-Aldrete, Wlodzimierz Bujalowski, Marc C. Morais, Erik Dill, Geoffrey S Diemer |
| المصدر: | Viruses, Vol 13, Iss 15, p 15 (2021) Viruses Volume 13 Issue 1 |
| بيانات النشر: | MDPI AG, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, viruses, ATPase, phi29, lcsh:QR1-502, P-loop ATPase, genome packaging, lcsh:Microbiology, Bacteriophage, 03 medical and health sciences, chemistry.chemical_compound, bacteriophage, ATP hydrolysis, Virology, Molecular motor, Homomeric, ASCE superfamily, asccphi28, 030102 biochemistry & molecular biology, biology, Chemistry, Lactococcus lactis, biochemical phenomena, metabolism, and nutrition, phage terminase, biology.organism_classification, Negative stain, dsDNA virus, molecular motor, encapsidation protein, 030104 developmental biology, Infectious Diseases, biology.protein, Biophysics, DNA, Macromolecule |
| الوصف: | Double-stranded DNA viruses package their genomes into pre-assembled protein procapsids. This process is driven by macromolecular motors that transiently assemble at a unique vertex of the procapsid and utilize homomeric ring ATPases to couple genome encapsidation to ATP hydrolysis. Here, we describe the biochemical and biophysical characterization of the packaging ATPase from Lactococcus lactis phage ascc&phi 28. Size-exclusion chromatography (SEC), analytical ultracentrifugation (AUC), small angle X-ray scattering (SAXS), and negative stain transmission electron microscopy (TEM) indicate that the ~45 kDa protein formed a 443 kDa cylindrical assembly with a maximum dimension of ~155 Å and radius of gyration of ~54 Å Together with the dimensions of the crystallographic asymmetric unit from preliminary X-ray diffraction experiments, these results indicate that gp11 forms a decameric D5-symmetric complex consisting of two pentameric rings related by 2-fold symmetry. Additional kinetic analysis shows that recombinantly expressed gp11 has ATPase activity comparable to that of functional ATPase rings assembled on procapsids in other genome packaging systems. Hence, gp11 forms rings in solution that likely reflect the fully assembled ATPases in active virus-bound motor complexes. Whereas ATPase functionality in other double-stranded DNA (dsDNA) phage packaging systems requires assembly on viral capsids, the ability to form functional rings in solution imparts gp11 with significant advantages for high-resolution structural studies and rigorous biophysical/biochemical analysis. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1999-4915 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::27eb58a07602c15c9e30fb06170ec404 https://www.mdpi.com/1999-4915/13/1/15 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....27eb58a07602c15c9e30fb06170ec404 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19994915 |
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