Cytosine deaminase suicide gene therapy for peritoneal carcinomatosis
| العنوان: | Cytosine deaminase suicide gene therapy for peritoneal carcinomatosis |
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| المؤلفون: | Chantal Lechanteur, Anne-Cécile Hellin, Georges Fillet, M. Lopez, Frédéric Princen, Marie-Paule Merville, Jacques Gielen, Vincent Bours, Mohamed Bentires-Alj |
| المصدر: | Cancer Gene Therapy. 7:20-26 |
| بيانات النشر: | Springer Science and Business Media LLC, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Male, Antimetabolites, Antineoplastic, Cancer Research, Genetic enhancement, Flucytosine, Apoptosis, Nucleoside Deaminases, Cytosine Deaminase, Tumor Cells, Cultured, Bystander effect, Animals, Humans, Medicine, Uridine, Molecular Biology, Peritoneal Neoplasms, business.industry, Carcinoma, Cytosine deaminase, Genetic Therapy, Transfection, Suicide gene, Molecular biology, In vitro, Rats, Disease Models, Animal, Cell culture, Feasibility Studies, Molecular Medicine, Colorectal Neoplasms, business, Neoplasm Transplantation |
| الوصف: | Gene therapy is a novel therapeutic approach that might soon improve the prognosis of some cancers. We investigated the feasibility of cytosine deaminase (CD) suicide gene therapy in a model of peritoneal carcinomatosis. DHD/K12 colorectal adenocarcinoma cells transfected in vitro with the CD gene were highly sensitive to 5-fluorocytosine (5-FC), and a bystander effect could also be observed. Treating CD+ cells with 5-FC resulted in apoptosis as detected by terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick-end labeling. In vitro, several human cell lines derived from ovarian or colorectal carcinomas, as well as the rat glioblastoma 9 L cell line, responded to CD/5-FC and showed a very strong bystander effect. 5-FC treatment of peritoneal carcinomatosis generated in syngeneic BDIX rats by CD-expressing DHD/K12 cells led to a complete and prolonged response and to prolonged survival. Our study thus demonstrated the efficacy of CD suicide gene therapy for the treatment of peritoneal carcinomatosis. |
| تدمد: | 1476-5500 0929-1903 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::283dcb6a165ab9a1279e172213ab999e https://doi.org/10.1038/sj.cgt.7700093 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....283dcb6a165ab9a1279e172213ab999e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14765500 09291903 |
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