Genome Assembly and Transcriptome Analysis of the Fungus Coniella diplodiella During Infection on Grapevine (Vitis vinifera L.)
| العنوان: | Genome Assembly and Transcriptome Analysis of the Fungus Coniella diplodiella During Infection on Grapevine (Vitis vinifera L.) |
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| المؤلفون: | Yiming Wang, Xiucai Fan, Chonghuai Liu, Lei Sun, Jianfu Jiang, Ying Zhang, Ruitao Liu, Peng Li |
| المصدر: | Frontiers in Microbiology, Vol 11 (2021) Frontiers in Microbiology |
| بيانات النشر: | Frontiers Media SA, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0106 biological sciences, Microbiology (medical), Hypersensitive response, lcsh:QR1-502, Nicotiana benthamiana, Secondary metabolite, 01 natural sciences, Microbiology, Genome, lcsh:Microbiology, Transcriptome, 03 medical and health sciences, medicine, pathogenicity, Coniella diplodiella, Gene, Original Research, 030304 developmental biology, Genetics, 0303 health sciences, biology, Effector, biology.organism_classification, grapevine, effector, genome assembly, transcriptome, 010606 plant biology & botany, medicine.drug |
| الوصف: | Grape white rot caused by Coniella diplodiella (Speg.) affects the production and quality of grapevine in China and other grapevine-growing countries. Despite the importance of C. diplodiella as a serious disease-causing agent in grape, the genome information and molecular mechanisms underlying its pathogenicity are poorly understood. To bridge this gap, 40.93 Mbp of C. diplodiella strain WR01 was de novo assembled. A total of 9,403 putative protein-coding genes were predicted. Among these, 608 and 248 genes are potentially secreted proteins and candidate effector proteins (CEPs), respectively. Additionally, the transcriptome of C. diplodiella was analyzed after feeding with crude grapevine leaf homogenates, which reveals the transcriptional expression of 9,115 genes. Gene ontology enrichment analysis indicated that the highly enriched genes are related with carbohydrate metabolism and secondary metabolite synthesis. Forty-three putative effectors were cloned from C. diplodiella, and applied for further functional analysis. Among them, one protein exhibited strong effect in the suppression of BCL2-associated X (BAX)-induced hypersensitive response after transiently expressed in Nicotiana benthamiana leaves. This work facilitates valuable genetic basis for understanding the molecular mechanism underlying C. diplodiella-grapevine interaction. |
| تدمد: | 1664-302X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2897fadac08376139db2e62d03b304bc https://doi.org/10.3389/fmicb.2020.599150 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2897fadac08376139db2e62d03b304bc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1664302X |
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