The relation between mitogen activated protein kinase (MAPK) pathway and different genes expression in patients with beta Thalassemia
| العنوان: | The relation between mitogen activated protein kinase (MAPK) pathway and different genes expression in patients with beta Thalassemia |
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| المؤلفون: | Asmaa A. Mahmoud, Mohamed Fa. Assar, Abdel Monem A Eltorgoman, Saher Elmasry, Yasser Elghobashy |
| المصدر: | Biochemistry and Biophysics Reports Biochemistry and Biophysics Reports, Vol 24, Iss, Pp 100836-(2020) |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, MAPK/ERK pathway, Thalassemia, Biophysics, Biochemistry, lcsh:Biochemistry, 03 medical and health sciences, 0302 clinical medicine, GNAI2, medicine, lcsh:QD415-436, lcsh:QH301-705.5, biology, Kinase, Beta thalassemia, β thalassemia, MAPK pathway, medicine.disease, Molecular biology, ARRB1, 030104 developmental biology, Real-time polymerase chain reaction, lcsh:Biology (General), 030220 oncology & carcinogenesis, Mitogen-activated protein kinase, biology.protein, Arrestin beta 1, GNAI2 and DUSP5, Research Article |
| الوصف: | Background β-thalassemia is an inherited hemoglobinopathy resulting in quantitative changes in the β-globin chain. Understanding the molecular basis of that disorder requires studying the expression of genes controlling the pathways that affect the erythropoietic homeostasis especially the MAPK pathway. The MAPKs are a family of serine/threonine kinases that play an essential role in connecting cell-surface receptors to DNA in the nucleus of the cell. Aim to study the effect of expression of GNAI2, DUSP5 and ARRB1 genes on MAPK signaling pathway in pediatric patients with beta thalassemia. Methods Forty children with beta thalassemia major (TM), forty children with beta thalassemia intermedia (TI) and forty age and gender matched healthy controls were enrolled in this study. Detection of GNAI2, DUSP5 and ARRB1 mRNA expression was done by real time polymerase chain reaction (RT-PCR). Results revealed increased expression of ARRB1 (Arrestin Beta 1) gene, and decreased expression of both GNAI2 (Guanine nucleotide-binding protein G (i) subunit alpha-2) and DUSP5 (Dual specificity protein phosphatase 5) genes in both patient groups than control groups respectively. Conclusions Change in the rate of expression of ARRB1, GNAI2 and DUSP5 may have a role in the pathogenesis of abnormal hematopoiesis in cases of β thalassemia through affecting the MAPK pathway. Highlights ∗ Β-thalassemia is an inherited hemoglobinopathy resulting in quantitative changes in the β-globin chain. ∗ Understanding the molecular basis of that disorder requires studying the expression of genes controlling the pathways that affect the erythropoietic homeostasis especially the MAPK pathway. ∗ The MAPKs are a family of serine/threonine kinases that play an essential role in connecting cell-surface receptors to DNA in the nucleus of the cell. ∗ Change in the rate of expression of ARRB1, GNAI2 and DUSP5 may have a role in the pathogenesis of abnormal hematopoiesis in cases of β thalassemia through affecting the MAPK pathway. |
| اللغة: | English |
| تدمد: | 2405-5808 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28b3d2148b285e7610f1fb86a9611355 http://europepmc.org/articles/PMC7644576 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....28b3d2148b285e7610f1fb86a9611355 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 24055808 |
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