Ceria nanoparticles with rhodamine B as a powerful theranostic agent against intracellular oxidative stress
| العنوان: | Ceria nanoparticles with rhodamine B as a powerful theranostic agent against intracellular oxidative stress |
|---|---|
| المؤلفون: | Hermenegildo García, Susana Rovira-Llopis, Herme G. Baldoví, José Raúl Herance, Sergio Navalón, Victor M. Victor, Nadezda Apostolova, Abdullah M. Asiri |
| المصدر: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname Repositori Universitat Jaume I Universitat Jaume I |
| بيانات النشر: | Royal Society of Chemistry, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Antioxidant, Biocompatibility, General Chemical Engineering, medicine.medical_treatment, Intracellular oxidative stress, macromolecular substances, medicine.disease_cause, THERAPY, NANOMEDICINE, HeLa, chemistry.chemical_compound, QUIMICA ORGANICA, Rhodamine B, medicine, GOLD, PROTECTION, OXIDE NANOPARTICLES, PHOTODEGRADATION, chemistry.chemical_classification, Reactive oxygen species, biology, Chemistry, LOCALIZATION, General Chemistry, biology.organism_classification, Biochemistry, PERSPECTIVES, Oxidative stress, Apoptosis, DISEASES, CELLS, Ceria nanoparticles, Intracellular, rhodamine B |
| الوصف: | Ceria nanoparticles with rhodamine B groups covalently attached on their surface (RhB-CeNPs) were successfully prepared to simultaneously exhibit antioxidant activity and the ability to detect oxidant species. In order to use them for biomedical purposes, the nanoparticles were internalized in two human cell lines (HeLa and Hep3B), confirmed by confocal microscopy. In addition, their biocompatibility was assessed by performing proliferation, viability and apoptosis assays, in which they showed a remarkable lack of toxicity. Thereafter, the antioxidant activity of RhB-CeNPs against reactive oxygen species (ROS) in a model of oxidative stress was demonstrated in HeLa cells using the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay. RhB-CeNPs exhibited higher cytosolic antioxidant activity than the well-established ceria nanoparticles. Surprisingly, the antioxidant capacity of RhB-CeNPs was evident when the ROS content of the cells increased notably (and was, therefore, harmful for those cells). Furthermore, the ability of RhB-CeNPs as ROS-content sensors was evaluated by measuring oxidative stress in HeLa cells using the intrinsic fluorescence of the rhodamine B groups present on the nanoparticles. The results with respect to the detection and quantification of ROS were similar to those obtained with DCFH-DA, a typical method of quantifying intracellular ROS. Our results demonstrate the potential of RhB-CeNPs as remarkably biocompatible theranostic agents with application in the field of oxidative stress. The present work was supported by the grant CP13/00252, PI13/1025 from Carlos III Health Institute, and by the European Regional Development Fund (ERDF). In addition, this study was financed by the Spanish Ministry of Economy and Competitiveness (Severo Ochoa and CTQ2012-32315), the Generalitat Valenciana (Prometeo 2012-013), Foundation for the Promotion of Health and Biomedical Research in the Valencian Region (UGP-14-095) and supported by the Spanish Ministry of Science and Innovation. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28f219c3158b0b245ec97a7d5986ab8a https://doi.org/10.1039/c5ra12794g |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....28f219c3158b0b245ec97a7d5986ab8a |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |