Prohibitin 1 (PHB1) controls growth and development and regulates proliferation and apoptosis in Schistosoma japonicum
| العنوان: | Prohibitin 1 (PHB1) controls growth and development and regulates proliferation and apoptosis in Schistosoma japonicum |
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| المؤلفون: | Hui-Fen Dong, Feng Ye, Yao-Dan Zhang, Mengjie Gu, Hong Jiang, Qin Ping Zhong, Wen-Jun Cheng, Rong Liu |
| المصدر: | The FASEB Journal. 34:11030-11046 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Virulence, Apoptosis, Real-Time Polymerase Chain Reaction, Biochemistry, Schistosoma japonicum, Mice, 03 medical and health sciences, 0302 clinical medicine, RNA interference, Prohibitins, parasitic diseases, Genetics, Animals, Prohibitin, Caenorhabditis elegans Proteins, Molecular Biology, Caenorhabditis elegans, Cell Proliferation, RNA, Double-Stranded, Schistosoma, Mice, Inbred BALB C, Gene knockdown, biology, Cell growth, fungi, Helminth Proteins, biology.organism_classification, Cell biology, Repressor Proteins, Fertility, 030104 developmental biology, Schistosomiasis japonica, Female, RNA Interference, 030217 neurology & neurosurgery, Biotechnology |
| الوصف: | Schistosomiasis is a zoonotic parasitic disease caused by the trematode blood flukes of the genus Schistosoma. The prodigious egg output of females is the main cause of the disease in definitive hosts, while the female worm relies on continuous pairing with the male worm to fuel the growth and maturation of the reproductive organs and egg production. Prohibitin, which contains the functionally interdependent PHB1 and PHB2 subunits in human and some other species, has been proposed to participate in the cell proliferation and apoptosis regulation in mammals. However, little is known about the function of PHB homolog in the growth and reproductive development of schistosomes. Here, we reported the Phb1 gene that was structurally and evolutionarily conserved in Schistosoma japonicum when compared with that of other species from Caenorhabditis elegans to human. Real-time PCR detected that SjPhb1 was highly transcribed in the vitellaria of female worms. SjPhb1 knockdown achieved through the dsRNA-mediated RNAi in vivo resulted in retarded growth, decreased pairing, and fecundity in adult worms, as well as attenuated pathogenicity or virulence of worms to their hosts. Cell proliferation and apoptosis examination found decreased cell proliferation and increased cell apoptosis in SjPhb1 dsRNA-treated worms. Therefore, our study provides the first characterization of S. japonicum PHB1 and reveals its fundamental role in the regulation of growth and development of S. japonicum by specific dsRNA-mediated RNAi in vivo. Our findings prompt for a promising molecular of schistosomes that can be targeted to effectively retard the growth and development of the schistosomes. |
| تدمد: | 1530-6860 0892-6638 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28fde663647ac4ae27f097bbff3efe59 https://doi.org/10.1096/fj.201902787rrr |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....28fde663647ac4ae27f097bbff3efe59 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15306860 08926638 |
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