Improvement in cryosurvival of buffalo bull (Bubalus bubalis) sperm by altering freezing rate within critical temperature range
| العنوان: | Improvement in cryosurvival of buffalo bull (Bubalus bubalis) sperm by altering freezing rate within critical temperature range |
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| المؤلفون: | Simarjeet Kaur, Ajeet Kumar, V. S. Malik, Sumit Singhal, Mrigank Honparkhe, Parkash Singh Brar, Jasmer Dalal, Pradeep Kumar |
| المصدر: | Asian Pacific Journal of Reproduction, Vol 7, Iss 2, Pp 72-78 (2018) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Sperm Head, lcsh:Medicine, Plant Science, Cryopreservation, Murrah buffalo, 03 medical and health sciences, Semen quality, 0302 clinical medicine, Animal science, Programmed freezing, medicine, Buffalo bulls, Freezing rate, 030219 obstetrics & reproductive medicine, General Veterinary, biology, Critical temperature range, Chemistry, lcsh:R, Obstetrics and Gynecology, Liquid nitrogen, biology.organism_classification, Bull semen, Sperm, 030104 developmental biology, Reproductive Medicine, Animal Science and Zoology, Swelling, medicine.symptom |
| الوصف: | Objective: To optimize the cryopreservation of buffalo bull semen by altering freezing rates within critical temperature range (4 °C to -60 °C). Methods: A total of 20 ejaculates each from 5 Murrah buffalo bulls were cryopreserved using programmable biofreezer in 2 phases. In the 1st phase, 9 freezing rates were applied at -2, -5, -10, -20, -30, -40, -50, -60 or -4 °C/min (control) from 4 °C to -15 °C ; at -40 °C/min from -15 °C to -60 °C. In the 2nd phase, a fixed freezing rate was applied at -30 °C /min from 4 °C to -15 °C. Six freezing rates were applied at -10, -20, -30, -40 (control), -50 or -60 °C/min from -15 °C to -60 °C. The freezing from -60 °C to -140 °C were fixed at -50 °C/min in both the phases. Post thaw semen quality was assessed in terms of motility, viability, membrane integrity (hypo-osmotic swelling test), sperm abnormalities, and active mitochondria. Data were arc sine transformed and analyzed through one-way analysis of variance using SPSS software. Results: In the 1st phase, percent individual motility, progressive motility and viability were similar among various protocols. Percent hypo-osmotic swelling reactive sperm was higher with freezing at -30 °C/min. In the 2nd phase, percent individual motility, viability and hypo-osmotic swelling reactive sperm was higher with freezing at -50 °C /min. Sperm head abnormalities were lower at -30 °C /min in the 1st phase, but were similar among the protocols of the 2nd phase. Percent active mitochondria were higher at -30 °C /min in the 1st phase and at -50 °C/min in the 2nd phase. Conclusions: The optimum post thaw semen quality of buffalo bull could be obtained by applying freezing rate at -30 °C/min (4 °C to -15 °C) and at -50 °C/min (-15 °C to -140 °C ), followed by plunging of straws in into liquid nitrogen for storage. |
| اللغة: | English |
| تدمد: | 2305-0519 2305-0500 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2943425cb071587030d44c222d799a00 http://www.apjr.net/article.asp?issn=2305-0500;year=2018;volume=7;issue=2;spage=72;epage=78;aulast=Dalal |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2943425cb071587030d44c222d799a00 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23050519 23050500 |
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