Rapid visualization of mammary gland tumor lesions of dogs using the enzyme-activated fluorogenic probe; γ-glutamyl hydroxymethyl rhodamine green
| العنوان: | Rapid visualization of mammary gland tumor lesions of dogs using the enzyme-activated fluorogenic probe; γ-glutamyl hydroxymethyl rhodamine green |
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| المؤلفون: | Yui, Hirose, Mona, Uchida, Masaya, Tsuboi, Takayuki, Nakagawa, Leo, Yaga, Shingo, Maeda, Yasuyuki, Momoi, Yugo, Kuriki, Mako, Kamiya, Yasuteru, Urano, Tomohiro, Yonezawa |
| المصدر: | Journal of Veterinary Medical Science. 84:593-599 |
| بيانات النشر: | Japanese Society of Veterinary Science, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Dogs, General Veterinary, Rhodamines, Animals, Female, Mammary Neoplasms, Animal, Dog Diseases, gamma-Glutamyltransferase, Fluorescent Dyes |
| الوصف: | Since gamma-glutamyl transpeptidase (GGT) is highly and locally expressed in human breast cancer, a GGT-enzymatically activatable fluorescent probe, gamma-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG), has been developed to detect the human breast cancer area with high performance. In this study, GGT expression and the efficacy of gGlu-HMRG on visualization were investigated in canine mammary gland tumors (MGT). Seventeen non-fixed fresh-frozen MGT specimens and each peritumoral control tissue were utilized. The GGT mRNA levels were highly observed in the tumor specimens compared with the control. GGT immunostaining was mostly observed on the cell membrane and cytosol of the alveolar and duct mammary epithelium of MGT tissues. These signals were strongly positive in several cases while they were mild to not observed in other cases. When gGlu-HMRG solution was dropped to the non-fixed tissue pieces of MGT or control tissues, the fluorescence intensities (FIs) were measured using Maestro in-vivo imaging device. FIs in MGT tissues were significantly higher than each control tissue 20 min after treatment. Based on Youden index method, the maximum sensitivity and specificity of FI was 82.4% and 82.4%. These findings suggest that GGT is highly expressed in several MGTs in dogs and gGlu-HMRG could visualize at least a part of MGT tissues in dogs. Nevertheless, it should be needed to assess the false-negative areas more carefully in canine than human cases. |
| تدمد: | 1347-7439 0916-7250 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::295f3bc503e0d93b3e45569faa26f423 https://doi.org/10.1292/jvms.22-0003 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....295f3bc503e0d93b3e45569faa26f423 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13477439 09167250 |
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