'All-in-One' Exosome Engineering Strategy for Effective Therapy of Familial Hypercholesterolemia
| العنوان: | 'All-in-One' Exosome Engineering Strategy for Effective Therapy of Familial Hypercholesterolemia |
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| المؤلفون: | Ruiyan Mei, Zhuo Wan, Zhelong Li, Mengying Wei, Weiwei Qin, Lijun Yuan, Li Liu, Guodong Yang |
| المصدر: | ACS Applied Materials & Interfaces. 14:50626-50636 |
| بيانات النشر: | American Chemical Society (ACS), 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Hyperlipoproteinemia Type II, Humans, RNA, General Materials Science, RNA, Messenger, Exosomes |
| الوصف: | Exosomes serve as a promising therapeutic nanoplatform. However, the exosomes produced by donor cells are a heterogeneous group, with only a small portion having high therapeutic efficacy. Specific isolation of the subpopulation with high efficacy is important for lowering the dose and minimizing toxicity. In this study, we loaded target mRNA and displayed specific Flag in engineered exosomes simultaneously. Briefly, the donor cells were transfected with plasmid expressing a fusion protein Flag-TCS-PTGFRN-CTSL-MCP, namely, exosome sorter. During biogenesis, the RNA-binding motif MCP can specifically bind with MS2-containing RNA and sort the target RNA into the lumen of exosomes. Anti-Flag magnetic beads can capture and thus purify the engineered exosomes |
| تدمد: | 1944-8252 1944-8244 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29a1612688f29b8db0056e0b377fcaf8 https://doi.org/10.1021/acsami.2c15785 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....29a1612688f29b8db0056e0b377fcaf8 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 19448252 19448244 |
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