Label-Free Detection of Rare Cell in Human Blood Using Gold Nano Slit Surface Plasmon Resonance
| العنوان: | Label-Free Detection of Rare Cell in Human Blood Using Gold Nano Slit Surface Plasmon Resonance |
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| المؤلفون: | Hsien-San Hou, Huai-Yi Chen, Steve R. Roffler, Chou-Yuan-Yuan Chang, Pei-Kuen Wei, Ji-Yen Cheng, Mansoureh Z. Mousavi |
| المصدر: | Biosensors Volume 5 Issue 1 Pages 98-117 Biosensors, Vol 5, Iss 1, Pp 98-117 (2015) |
| بيانات النشر: | MDPI AG, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Rare cell, magnetic nanoparticles, Materials science, lcsh:Biotechnology, Clinical Biochemistry, Metal Nanoparticles, Nanotechnology, Biosensing Techniques, microfluidic chip, Article, double capture, lcsh:TP248.13-248.65, Lab-On-A-Chip Devices, Nano, Humans, Surface plasmon resonance, Label free, Blood Cells, Human blood, gold nanoslits, General Medicine, Surface Plasmon Resonance, rare cells, Slit, Microfluidic chip, Magnetic nanoparticles, Gold, Blood Chemical Analysis |
| الوصف: | Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications and various fields of research, such as detection of circulating tumor cells for cancer therapy and stem cells studies. Surface Plasmon Resonance (SPR) as a label-free method is a promising technology for detection of rare cells for diagnosis or research applications. Short detection depth of SPR (400 nm) provides a sensitive method with minimum interference of non-targets in the biological samples. In this work, we developed a novel microfluidic chip integrated with gold nanoslit SPR platform for highly efficient immunomagnetic capturing and detection of rare cells in human blood. Our method offers simple yet efficient detection of target cells with high purity. The approach for detection consists of two steps. Target cells are firs captured on functionalized magnetic nanoparticles (MNPs) with specific antibody I. The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film. MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area. The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits. |
| وصف الملف: | application/pdf |
| تدمد: | 2079-6374 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29d225c3f8f3502744feeb9eeb7b8e09 https://doi.org/10.3390/bios5010098 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....29d225c3f8f3502744feeb9eeb7b8e09 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20796374 |
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