A 'Clickable' Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes
| العنوان: | A 'Clickable' Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes |
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| المؤلفون: | Richard J. Karpowicz, David M. Chenoweth, Conor M. Haney, E. James Petersson, Virginia M.-Y. Lee, Sam Giannakoulias, Joomyung V. Jun |
| المصدر: | Journal of the American Chemical Society. 141:1893-1897 |
| بيانات النشر: | American Chemical Society (ACS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Conjugated system, 010402 general chemistry, Tracking (particle physics), 01 natural sciences, Biochemistry, Article, Catalysis, Small Molecule Libraries, Colloid and Surface Chemistry, Molecule, Fluorescent Dyes, chemistry.chemical_classification, Aza Compounds, Molecular Structure, Chemistry, Biomolecule, Vesicle, fungi, food and beverages, General Chemistry, Photochemical Processes, Fluorescence, Small molecule, 0104 chemical sciences, alpha-Synuclein, Click chemistry, Biophysics, Click Chemistry |
| الوصف: | Photoconvertible fluorophores can enable the visualization and tracking of a specific biomolecules, complexes, and cellular compartments with precise spatiotemporal control. The field of photoconvertible probes is dominated by fluorescent protein variants, which can introduce perturbations to the target biomolecules due to their large size. Here, we present a photoconvertible small molecule, termed CPX, that can be conjugated to any target through azide-alkyne cycloaddition ("click" reaction). To demonstrate its utility, we have applied CPX to study (1) trafficking of biologically relevant synthetic vesicles and (2) intracellular processes involved in transmission of α-synuclein (αS) pathology. Our results demonstrate that CPX can serve as a minimally perturbing probe for tracking the dynamics of biomolecules. |
| تدمد: | 1520-5126 0002-7863 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29e6d523311e503fe75c3d68b72dd3fb https://doi.org/10.1021/jacs.8b13094 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....29e6d523311e503fe75c3d68b72dd3fb |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15205126 00027863 |
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