Different decellularization methods in bovine lung tissue reveals distinct biochemical composition, stiffness, and viscoelasticity in reconstituted hydrogels
| العنوان: | Different decellularization methods in bovine lung tissue reveals distinct biochemical composition, stiffness, and viscoelasticity in reconstituted hydrogels |
|---|---|
| المؤلفون: | Alican Kuşoğlu, Kardelen Yangın, Sena N. Özkan, Sevgi Sarıca, Deniz Örnek, Nuriye Solcan, İsmail C. Karaoğlu, Seda Kızılel, Pınar Bulutay, Pınar Fırat, Suat Erus, Serhan Tanju, Şükrü Dilege, Ece Öztürk |
| المساهمون: | Öztürk, Ece (ORCID 0000-0001-8635-0279 & YÖK ID 326940), Kuşoğlu, Alican, Özkan, Sena Nur, Sarıca, Sevgi, Örnek, Deniz, Yangın, Kardelen, Solcan, Nuriye, Karaoğlu, İsmail Can, Kızılel, Seda (ORCID 0000-0001-9092-2698 & YÖK ID 28376), Bulutay, Pınar (ORCID 0000-0001-5497-1513 & YÖK ID 133565), Fırat, Pınar Arıkan (ORCID 0000-0001-8340-2678 & YÖK ID 207545), Erus, Suat (ORCID 0000-0002-6162-3266 & YÖK ID 175565), Tanju, Serhan (ORCID 0000-0002-2363-233X & YÖK ID 214690), Dilege, Şükrü (ORCID 0000-0002-1071-5291 & YÖK ID 122573), Koç University Research Center for Translational Medicine (KUTTAM) / Koç Üniversitesi Translasyonel Tıp Araştırma Merkezi (KUTTAM), School of Medicine, Graduate School of Health Sciences, Graduate School of Sciences and Engineering, College of Engineering |
| المصدر: | ACS Applied Bio Materials |
| بيانات النشر: | American Chemical Society (ACS), 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Biomaterials, Decellularization, Lung hydrogels, Tissue engineering, Lung cancer, Extracellular matrix, Biochemistry (medical), Biomedical Engineering, General Chemistry, Nanoscience and nanotechnology, Materials science, biomaterials |
| الوصف: | Extracellula r matri x (ECM)-derived hydrogels are in demand for use in lung tissue engineering to mimic the native microenvironment of cells in vitro. Decellularization of native tissues has been pursued for preser v i n g organotypic ECM while eliminating cellular content and reconstitution into scaffolds which allows re-cellularization for modeling homeostasis, regeneration, or diseases. Achieving mechanical stabi l i t y and understanding the effects of the decellularization process on mechanical parameters of the reconstituted ECM hydrogels present a challenge in the field. Stiffness and viscoelasticity are important characteristics of tissue mechanics that regulate crucial cellular processes and their in vitro representation in engineered models is a current aspiration. The effect of decellulariza-tion on viscoelastic properties of resulting ECM hydrogels has not yet been addressed. The aim of this study was to establish bovine lung tissue decellularization for the first time via pursuing four different protocols and characterization of reconstituted decellularized lung ECM hydrogels for biochemical and mechanical properties. Our data reveal that bovine lungs provide a reproducible alternative to human lungs for disease modeling with optimal retention of ECM components upon decellularization. We demonstrate that the decellularization method significa n t l y affects ECM content, stiffness, and viscoelastic properties of resulting hydrogels. Lastly, we examined the impact of these aspects on viabi l i t y , morphology, and growth of lung cancer cells, healthy bronchial epithelial cells, and patient-derived lung organoids. This work was funded by the International Fellowship for Outstanding Researchers Program of Scientific and Technological Research Council of Turkey (TÜBİTAK) (grant no. 118C238) and Marie Sklodowska-Curie Individual Fellowship (MiTuMi, grant no. 101032602). |
| وصف الملف: | |
| اللغة: | English |
| تدمد: | 1010-3260 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a048d63f2ba4854fee1dd3b69fa8080 http://cdm21054.contentdm.oclc.org/cdm/ref/collection/IR/id/10989 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2a048d63f2ba4854fee1dd3b69fa8080 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10103260 |
|---|