Predicting Irreparable Renal Ischemic Injury Using a Real-Time Marker in the Porcine Model
| العنوان: | Predicting Irreparable Renal Ischemic Injury Using a Real-Time Marker in the Porcine Model |
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| المؤلفون: | Harvey A. Schwertner, Donna M. Hensley, R. Duane Cespedes, Jerry R Cowart, Kyle J. Weld, Patricia S. Dixon, Anneke C. Bush, Claudio Montiglio |
| المصدر: | Journal of Urology. 180:2218-2225 |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Blood Glucose, Glycerol, Nephrology, medicine.medical_specialty, Pathology, Swine, Urology, Urinary system, Ischemia, Renal function, Kidney, Kidney Function Tests, Nephrectomy, Sensitivity and Specificity, Random Allocation, chemistry.chemical_compound, Internal medicine, medicine, Animals, Warm Ischemia, cardiovascular diseases, Pyruvates, Probability, Analysis of Variance, Creatinine, Warm Ischemia Time, business.industry, medicine.disease, Disease Models, Animal, medicine.anatomical_structure, chemistry, Reperfusion Injury, Lactates, Female, business, Biomarkers, Glomerular Filtration Rate, Kidney disease |
| الوصف: | We determined the maximal renal tolerance of warm ischemia using renal cortical interstitial metabolic changes to identify a potential real-time marker of irreparable renal function.Using a single kidney model 3 groups of 5 pigs each underwent 120, 150 and 180 minutes of warm ischemia, respectively. Microdialysis samples were collected before, during and after ischemia. Renal function assessments consisting of serum creatinine and GFR measurements were performed before ischemia and on post-ischemia days 1, 5, 9, 14 and 28. Kidneys exposed and not exposed to ischemia were collected for histological study.Interstitial glucose and pyruvate concentrations decreased, while lactate concentrations increased to stable levels during ischemia. Glutamate spiked at 30 minutes of ischemia and subsequently tapered, while glycerol increased throughout warm ischemia time. At post-ischemia day 28 renal function returned to pre-ischemia baseline levels in the group with 120 minutes of ischemia but did not recover to baseline in the 150 and 180-minute ischemic groups. Functional data correlated with histological findings. The 120-minute maximal renal tolerance of warm ischemia correlated with a mean +/- SD glycerol concentration of 167 +/- 24 micromol/l.Interstitial glycerol is a real-time, renal unit specific, minimally invasive marker of renal function deterioration. Exposure of porcine kidneys to ischemic insults resulting in renal cortical interstitial glycerol concentrations higher than 167 micromol/l is associated with irreparable functional damage in this model. |
| تدمد: | 1527-3792 0022-5347 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a24a658357267933a2411c46e3cafbc https://doi.org/10.1016/j.juro.2008.07.017 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....2a24a658357267933a2411c46e3cafbc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15273792 00225347 |
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