Highly specific and selective anti-pS396-tau antibody C10.2 targets seeding-competent tau
| العنوان: | Highly specific and selective anti-pS396-tau antibody C10.2 targets seeding-competent tau |
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| المؤلفون: | Karsten Skjødt, Jan Egebjerg, Jan Torleif Pedersen, Fredrik Kartberg, Christiane Volbracht, Florence Sotty, Nina Helen Rosenqvist, Lars Østergaard Pedersen, Einar M. Sigurdsson, Ayodeji A. Asuni, Pia Jul, Christian Rungsted Andersson, Jeppe Falsig, Lone Helboe, Jeffrey B. Stavenhagen, Justus Claus Alfred Daechsel, Søren Christensen |
| المصدر: | Alzheimer's & Dementia : Translational Research & Clinical Interventions Rosenqvist, N, Asuni, A A, Andersson, C R, Christensen, S, Daechsel, J A, Egebjerg, J, Falsig, J, Helboe, L, Jul, P, Kartberg, F, Pedersen, L Ø, Sigurdsson, E M, Sotty, F, Skjødt, K, Stavenhagen, J B, Volbracht, C & Pedersen, J T 2018, ' Highly specific and selective anti-pS396-tau antibody C10.2 targets seeding-competent tau ', Alzheimer's & Dementia: Translational Research & Clinical Interventions, vol. 4, pp. 521-534 . https://doi.org/10.1016/j.trci.2018.09.005 |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Monoclonal antibody, medicine.drug_class, Tau protein, Hyperphosphorylation, Anti-tau antibodies, C10.2, Peptide, D1.2, 03 medical and health sciences, 0302 clinical medicine, C8.3, Tau seeding, mental disorders, medicine, IC50, chemistry.chemical_classification, biology, HEK 293 cells, Neurodegeneration, Featured Article, medicine.disease, Phospho-serine 396, C5.2, Cell biology, Psychiatry and Mental health, 030104 developmental biology, chemistry, biology.protein, Neurology (clinical), Antibody, 030217 neurology & neurosurgery |
| الوصف: | Introduction: The abnormal hyperphosphorylation of the microtubule-associated protein tau plays a crucial role in neurodegeneration in Alzheimer's disease (AD) and other tauopathies.Methods: Highly specific and selective anti-pS396-tau antibodies have been generated using peptide immunization with screening against pathologic hyperphosphorylated tau from rTg4510 mouse and AD brains and selection in in vitro and in vivo tau seeding assays.Results: The antibody C10.2 bound specifically to pS396-tau with an IC50 of 104 pM and detected preferentially hyperphosphorylated tau aggregates from AD brain with an IC50 of 1.2 nM. C10.2 significantly reduced tau seeding of P301L human tau in HEK293 cells, murine cortical neurons, and mice. AD brain extracts depleted with C10.2 were not able to seed tau in vitro and in vivo, demonstrating that C10.2 specifically recognized pathologic seeding-competent tau.Discussion: Targeting pS396-tau with an antibody like C10.2 may provide therapeutic benefit in AD and other tauopathies. |
| وصف الملف: | application/pdf |
| تدمد: | 2352-8737 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a4e509d5c60e3458f1a368b67631b5a https://pubmed.ncbi.nlm.nih.gov/30386817 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2a4e509d5c60e3458f1a368b67631b5a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23528737 |
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