Auto-inhibitory effects of an IQ motif on protein structure and function

التفاصيل البيبلوغرافية
العنوان: Auto-inhibitory effects of an IQ motif on protein structure and function
المؤلفون: Joachim Behlke, Sandro Keller, Hannelore Haase, Janine Lossie, Daria Petzhold, Ingo Morano
المصدر: Biochemical and Biophysical Research Communications. 396:939-943
بيانات النشر: Elsevier BV, 2010.
سنة النشر: 2010
مصطلحات موضوعية: Circular dichroism, Myosin Light Chains, Myosin light-chain kinase, Biophysics, macromolecular substances, Biochemistry, Protein–protein interaction, Myosin head, Protein structure, Myosin, Animals, Molecular Biology, Sequence Deletion, Adenosine Triphosphatases, Myosin Type II, chemistry.chemical_classification, Alanine, Myosin Heavy Chains, Circular Dichroism, Cell Biology, Surface Plasmon Resonance, Protein Structure, Tertiary, Rats, Amino acid, chemistry, Ultracentrifugation
الوصف: The denuded IQ2 domain, i.e. myosin heavy chain not associated with regulatory light chains, exerts an inhibitory effect on myosin ATPase activity. In this study, we elaborated a structural explanation for this auto-inhibitory effect of IQ2 on myosin function. We employed analytical ultracentrifugation, circular dichroism, and surface plasmon resonance spectroscopy to investigate structural and functional properties of a myosin heavy chain (MYH) head-rod fragment aa664–915. MYH664–915 was monomeric, adopted a closed shape, and bound essential myosin light chains (HIS-MLC-1) with low affinity to IQ1. Deletion of IQ2, however opened MYH664–915. Four amino acids present in IQ2 could be identified to be responsible for this auto-inhibitory structural effect: alanine mutagenesis of I814, Q815, R819, and W827 stretched MYH664–915 and increased 30-fold the binding affinity of HIS-MLC-1 to IQ1. In this study we show, that denuded IQ2 favours a closed conformation of myosin with a low HIS-MLC-1 binding affinity. The collapsed structure of myosin with denuded IQ2 could explain the auto-inhibitory effects of IQ2 on enzymatic activity of myosin.
تدمد: 0006-291X
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2a814ff2ea724b98f83bcc30932b602d
https://doi.org/10.1016/j.bbrc.2010.05.027
حقوق: OPEN
رقم الأكسشن: edsair.doi.dedup.....2a814ff2ea724b98f83bcc30932b602d
قاعدة البيانات: OpenAIRE