Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticus
| العنوان: | Selection and characterization of ssDNA aptamers specifically recognizing pathogenic Vibrio alginolyticus |
|---|---|
| المؤلفون: | Huizhi Mi, S. Wu, Xianling Qin, Mingzhu Liu, Pengfei Li, Qing Yu, Siqiao Li, Deqiang Shi, Zijun Lu, Hongfei Su, Hehe Xiao |
| المصدر: | Journal of Fish Diseases. 42:851-858 |
| بيانات النشر: | Wiley, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0301 basic medicine, Veterinary (miscellaneous), Aptamer, DNA, Single-Stranded, Aquatic Science, Biology, Ligands, Sensitivity and Specificity, Fish Diseases, 03 medical and health sciences, chemistry.chemical_compound, Animals, Pathogen, Vibrio alginolyticus, Fishes, 04 agricultural and veterinary sciences, Aptamers, Nucleotide, Flow Cytometry, biology.organism_classification, In vitro, 030104 developmental biology, chemistry, Biochemistry, Vibrio Infections, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Molecular probe, DNA, Systematic evolution of ligands by exponential enrichment, Bacteria |
| الوصف: | Vibrio alginolyticus (V. alginolyticus) is a major opportunistic pathogen to both marine animals and humans, which has also caused heavy economic losses to mariculture. The aim of this study was to develop highly specific aptamers for V. alginolyticus. Single-stranded DNA (ssDNA) aptamers with high binding affinity to viable V. alginolyticus were generated by Systematic Evolution of Ligands by Exponential Enrichment (SELEX) and identified by flow cytometric analysis in this study. The selected aptamers showed high specificity for V. alginolyticus and low apparent binding for other bacteria. The aptamers formed distinct stem-loop structures, which could form the basis of aptamers' specific binding to the target V. alginolyticus. Aptamer VA2 and VA8 showed particularly high binding affinity constant (Kd) of 14.31 ± 4.26 and 90.00 ± 13.51 nM, respectively. The aptamers produced no cytotoxic effects in vitro and in vivo. ssDNA aptamers were successfully selected against the viable bacteria pathogen V. alginolyticus by SELEX. The aptamers selected in this study could be not only applied as specific chemical molecular probes for studying V. alginolyticus pathogenesis to Trachinotus ovatus, but also developing rapid convenient diagnosis assay for V. alginolyticus infection, even when applied to the complex sample matrix, such as food and environment samples. |
| تدمد: | 1365-2761 0140-7775 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2aad268f7c0bc6d2d3e70c199734a53a https://doi.org/10.1111/jfd.12985 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2aad268f7c0bc6d2d3e70c199734a53a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652761 01407775 |
|---|