Insights into the epithelial mesenchymal transition phenotype in cancer of unknown primary from a global microRNA profiling study
| العنوان: | Insights into the epithelial mesenchymal transition phenotype in cancer of unknown primary from a global microRNA profiling study |
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| المؤلفون: | Stoyianni, A., Pentheroudakis, George, Benjamin, H., Cervantes, A., Ashkenazi, K., Lazaridis, G., Pavlidis, Nicholas, Spector, Y. |
| المساهمون: | Pavlidis, Nicholas [0000-0002-2195-9961], Pentheroudakis, George [0000-0002-6632-2462] |
| المصدر: | Clinical and Translational Oncology |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Male, Cancer Research, Pathology, Unclassified drug, Cell, Microrna, Metastasis, Tissue microarray, Nerve cell adhesion molecule, Neoplasms, 80 and over, Overall survival, Middle aged, Aged, 80 and over, Unknown primary, General Medicine, Middle Aged, Epithelial-mesenchymal transition, Microrna 203, Immunohistochemistry, Phenotype, medicine.anatomical_structure, Oncology, embryonic structures, Female, Cancer chemotherapy, Uvomorulin, Human, Adult, medicine.medical_specialty, Epithelial-Mesenchymal Transition, Histopathology, Major clinical study, Biology, Article, Cancer of unknown primary, Transcription factor snail, Microrna 205, Downregulation and upregulation, Upregulation, microRNA, Genetics, medicine, Humans, Epithelial–mesenchymal transition, Human tissue, Aged, Cancer of unknown primary site, Very elderly, Microarray analysis, Histology, Microrna 141, Microrna 200a, Microarray Analysis, medicine.disease, Cancer survival, Microrna 200c, Micrornas, Microrna 200b, MicroRNAs, Human cell, Gene chip analysis, Protein expression, Neoplasms, Unknown Primary, Epithelial mesenchymal transition |
| الوصف: | Purpose: We sought to study the microRNA regulation of epithelial mesenchymal transition (EMT), the acquisition of migratory, mesenchymal-like properties of epithelial cells, in cancer of unknown primary (CUP). Patients and methods: We studied the global expression profile of 982 microRNAs by means of microarray technology in 68 CUP cases immunohistochemically characterised as EMT-positive (n = 5 by % of cells or n = 10 by a semiquantitative H-score) or EMT-negative. Results: EMT-suppressive miRNAs such as miR-203 and members of the miR-200 family (miR-200a,b,c and miR-141) presented a 2.45 to 3.64-fold lower expression level in the EMT-positive cases without, however, reaching statistical significance. MiR-205, a squamous tissue-specific marker, was very variable in the data set. Excluding CUP cases with squamous cell histology, miR-205, miR-203 and the miR-200 family exhibited a trend of downregulation in EMT-positive cases. A similar pattern of miRNA expression was detected when the comparison took place between EMT-positive vs EMT-negative cases according to the H-score. Moreover, miR-203, miR-205 and miR-200c were numerically downregulated in those tumours with high expression of the EMT marker N-cadherin. Conclusions: The EMT-suppressive miR-203 and miR-200 family were consistently but non-significantly downregulated in CUP with the EMT phenotype. A larger study is warranted to further explore the role of microRNAs in CUP. © 2013 Federación de Sociedades Españolas de Oncología (FESEO). 16 8 725 731 |
| تدمد: | 1699-3055 1699-048X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b3f506efc1c6dfafbce95f71dc8d2ba https://doi.org/10.1007/s12094-013-1139-5 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2b3f506efc1c6dfafbce95f71dc8d2ba |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16993055 1699048X |
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