Development of safe and highly protective live-attenuated SARS-CoV-2 vaccine candidates by genome recoding
| العنوان: | Development of safe and highly protective live-attenuated SARS-CoV-2 vaccine candidates by genome recoding |
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| المؤلفون: | Volker Thiel, Tran Thi Nhu Thao, Julia Adler, Theresa C. Firsching, Jakob Trimpert, Nikolaus Osterrieder, Joerg Jores, Fabien Labroussaa, Susanne Kaufer, Dusan Kunec, Thomas Höfler, Achim D. Gruber, Azza Abdelgawad, Kristina Dietert, Nadine Ebert, Daria Vladimirova, Luca D. Bertzbach, Andelé M. Conradie |
| المصدر: | Cell Reports Trimpert, Jakob; Dietert, Kristina; Firsching, Theresa C; Ebert, Nadine; Tran, Thi Nhu Thao; Vladimirova, Daria; Kaufer, Susanne; Labroussaa, Fabien; Abdelgawad, Azza; Conradie, Andelé; Höfler, Thomas; Adler, Julia M; Bertzbach, Luca D; Jores, Jörg; Gruber, Achim D; Thiel, Volker; Osterrieder, Nikolaus; Kunec, Dusan (2021). Development of safe and highly protective live-attenuated SARS-CoV-2 vaccine candidates by genome recoding. Cell reports, 36(5), p. 109493. Cell Press 10.1016/j.celrep.2021.109493 <http://dx.doi.org/10.1016/j.celrep.2021.109493> |
| بيانات النشر: | Freie Universit��t Berlin, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | synthetic attenuated virus engineering, COVID-19 Vaccines, Syrian hamster, viruses, Respiratory System, coronavirus, 610 Medicine & health, Genome, Viral, Disease, Roborovski dwarf hamster, Vaccines, Attenuated, Virus Replication, medicine.disease_cause, Article, General Biochemistry, Genetics and Molecular Biology, Virus, Immunity, Chlorocebus aethiops, Pandemic, medicine, Animals, Humans, codon pair deoptimization, Pandemics, Vero Cells, Coronavirus, Gene Editing, live attenuated vaccine, Attenuated vaccine, 630 Agriculture, Mesocricetus, biology, business.industry, SARS-CoV-2, COVID-19, 500 Naturwissenschaften und Mathematik::570 Biowissenschaften, Biologie::570 Biowissenschaften, Biologie, biology.organism_classification, Virology, Viral replication, genome recoding, Mutation, 570 Life sciences, 590 Animals (Zoology), business |
| الوصف: | Safe and effective vaccines are urgently needed to stop the pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We construct a series of live attenuated vaccine candidates by large-scale recoding of the SARS-CoV-2 genome and assess their safety and efficacy in Syrian hamsters. Animals were vaccinated with a single dose of the respective recoded virus and challenged 21 days later. Two of the tested viruses do not cause clinical symptoms but are highly immunogenic and induce strong protective immunity. Attenuated viruses replicate efficiently in the upper but not in the lower airways, causing only mild pulmonary histopathology. After challenge, hamsters develop no signs of disease and rapidly clear challenge virus: at no time could infectious virus be recovered from the lungs of infected animals. The ease with which attenuated virus candidates can be produced and administered favors their further development as vaccines to combat the ongoing pandemic. Graphical abstract Trimpert et al. construct safe and efficacious live attenuated SARS-CoV-2 vaccine candidates by recoding the SARS-CoV-2 genome. The lead vaccine candidate, sCPD9, is apathogenic in Syrian and Roborovski dwarf hamsters. A single intranasal droplet vaccination with sCPD9 induces strong neutralizing antibody responses and protects hamsters from disease caused by SARS-CoV-2. |
| وصف الملف: | application/pdf |
| DOI: | 10.17169/refubium-32320 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b41ad2a1d3cb7ccdca98766f39f79ba |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2b41ad2a1d3cb7ccdca98766f39f79ba |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.17169/refubium-32320 |
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