Biochemical Validation of a Second Guanidine Riboswitch Class in Bacteria
| العنوان: | Biochemical Validation of a Second Guanidine Riboswitch Class in Bacteria |
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| المؤلفون: | Ronald R. Breaker, Madeline E. Sherlock, Sarah N. Malkowski |
| المصدر: | Biochemistry. 56:352-358 |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Riboswitch, Aptamer, Computational biology, Biology, Cyanobacteria, Ligands, Guanidines, Biochemistry, Article, Antiporters, Bacterial genetics, 03 medical and health sciences, chemistry.chemical_compound, Escherichia coli, Guanidine, Gene, Regulation of gene expression, 030102 biochemistry & molecular biology, Escherichia coli Proteins, Computational Biology, RNA, Gene Expression Regulation, Bacterial, Aptamers, Nucleotide, Klebsiella pneumoniae, RNA, Bacterial, 030104 developmental biology, chemistry, Cobalamin riboswitch, Nucleic Acid Conformation |
| الوصف: | Recently, it was determined that representatives of the riboswitch candidates called ykkC and ykkC-III directly bind free guanidine. Guanidine-binding ykkC motif RNAs, now renamed guanidine-I riboswitches, were demonstrated to commonly regulate the expression of genes encoding guanidine carboxylases, as well as others encoding guanidine efflux proteins such as EmrE and SugE. Likewise, genes encoding similar efflux proteins are associated with ykkC-III motif RNAs, which have now been renamed guanidine-III riboswitches. Prior to the validation of guanidine as the ligand for these newly established riboswitch classes, another RNA motif was discovered by comparative genomic analysis and termed mini-ykkC because of its small size and gene associations similar to those of the original ykkC motif. It was hypothesized that these distinct RNA structures might respond to the same ligand. However, the small size and repetitive nature of mini-ykkC RNAs suggested that it might respond to ligand via the action of a protein factor. Herein, we demonstrate that, despite its extremely simple architecture, mini-ykkC motif RNAs constitute a distinct class of guanidine-sensing RNAs, called guanidine-II riboswitches. Surprisingly, each of the two stem-loop structures that comprise the mini-ykkC motif appears to directly bind free guanidine in a cooperative manner. These findings reveal that bacteria make extensive use of diverse guanidine-responsive riboswitches to overcome the toxic effects of this compound. |
| تدمد: | 1520-4995 0006-2960 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b5b60d99f16cfe13049d1c91d39cf18 https://doi.org/10.1021/acs.biochem.6b01270 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2b5b60d99f16cfe13049d1c91d39cf18 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15204995 00062960 |
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