Mycobacterium chelonae Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report
| العنوان: | Mycobacterium chelonae Infection Identified by Metagenomic Next-Generation Sequencing as the Probable Cause of Acute Contained Rupture of a Biological Composite Graft—A Case Report |
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| المؤلفون: | Andrea C. Büchler, Vladimir Lazarevic, Nadia Gaïa, Myriam Girard, Friedrich Eckstein, Adrian Egli, Sarah Tschudin Sutter, Jacques Schrenzel |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 381, p 381 (2022) International Journal of Molecular Sciences |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | clinical metagenomics, Mycobacteroides, mycobacteria, QH301-705.5, Organic Chemistry, aortic surgery, Case Report, General Medicine, shotgun sequencing, Catalysis, Computer Science Applications, Inorganic Chemistry, Chemistry, Physical and Theoretical Chemistry, Biology (General), culture-negative infection, Molecular Biology, QD1-999, Spectroscopy |
| الوصف: | We present the case of a 72-year-old female patient with acute contained rupture of a biological composite graft, 21 months after replacement of the aortic valve and the ascending aorta due to an aortic dissection. Auramine-rhodamine staining of intraoperative biopsies showed acid-fast bacilli, but classical culture and molecular methods failed to identify any organism. Metagenomic analysis indicated infection with Mycobacterium chelonae, which was confirmed by target-specific qPCR. The complexity of the sample required a customized bioinformatics pipeline, including cleaning steps to remove sequences of human, bovine ad pig origin. Our study underlines the importance of multiple testing to increase the likelihood of pathogen identification in highly complex samples. |
| اللغة: | English |
| تدمد: | 1661-6596 1422-0067 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b6b3a59d55d58acd6d1356061c57487 https://www.mdpi.com/1422-0067/23/1/381 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2b6b3a59d55d58acd6d1356061c57487 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16616596 14220067 |
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