BRG1 co-localizes with DNA replication factors and is required for efficient replication fork progression
| العنوان: | BRG1 co-localizes with DNA replication factors and is required for efficient replication fork progression |
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| المؤلفون: | Paul D. Chastain, David G. Kaufman, Bernard E. Weissman, Scott J. Bultman, Stephanie M. Cohen, Gary B. Rosson, Beezly S. Groh |
| المصدر: | Nucleic Acids Research |
| بيانات النشر: | Oxford University Press, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | DNA Replication, Embryonic Development, Eukaryotic DNA replication, Biology, Genome Integrity, Repair and Replication, Pre-replication complex, 03 medical and health sciences, Mice, 0302 clinical medicine, Replication factor C, Minichromosome maintenance, Control of chromosome duplication, Erythroid Cells, Genetics, Animals, Humans, 030304 developmental biology, Cell Proliferation, 0303 health sciences, DNA Helicases, Nuclear Proteins, DNA Replication Fork, Molecular biology, Chromatin, DNA-Binding Proteins, Licensing factor, Phenotype, 030220 oncology & carcinogenesis, Mutation, Origin recognition complex, HeLa Cells, Transcription Factors |
| الوصف: | For DNA replication to occur, chromatin must be remodeled. Yet, we know very little about which proteins alter nucleosome occupancy at origins and replication forks and for what aspects of replication they are required. Here, we demonstrate that the BRG1 catalytic subunit of mammalian SWI/SNF-related complexes co-localizes with origin recognition complexes, GINS complexes, and proliferating cell nuclear antigen at sites of DNA replication on extended chromatin fibers. The specific pattern of BRG1 occupancy suggests it does not participate in origin selection but is involved in the firing of origins and the process of replication elongation. This latter function is confirmed by the fact that Brg1 mutant mouse embryos and RNAi knockdown cells exhibit a 50% reduction in replication fork progression rates, which is associated with decreased cell proliferation. This novel function of BRG1 is consistent with its requirement during embryogenesis and its role as a tumor suppressor to maintain genome stability and prevent cancer. |
| اللغة: | English |
| تدمد: | 1362-4962 0305-1048 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ba30a27d87c3289650b956781757e42 http://europepmc.org/articles/PMC2978342 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2ba30a27d87c3289650b956781757e42 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13624962 03051048 |
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