Metabolomic Analysis of Small Extracellular Vesicles Derived from Pancreatic Cancer Cells Cultured under Normoxia and Hypoxia
| العنوان: | Metabolomic Analysis of Small Extracellular Vesicles Derived from Pancreatic Cancer Cells Cultured under Normoxia and Hypoxia |
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| المؤلفون: | Tomoyoshi Soga, Sho Tabata, Masaru Mori, Masako Hasebe, Satsuki Ikeda, Akiyoshi Hirayama, Ryosuke Hayasaka, Masaru Tomita, Sumiko Ohnuma |
| المصدر: | Metabolites Volume 11 Issue 4 Metabolites, Vol 11, Iss 215, p 215 (2021) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, metabolome analysis, Angiogenesis, Endocrinology, Diabetes and Metabolism, pancreatic cancer, lcsh:QR1-502, small extracellular vesicles, supercritical fluid chromatography-tandem mass spectrometry, Biochemistry, Article, lcsh:Microbiology, Metastasis, 03 medical and health sciences, 0302 clinical medicine, Metabolomics, Pancreatic cancer, microRNA, medicine, Molecular Biology, liquid chromatography-mass spectrometry, capillary ion chromatography-mass spectrometry, Tumor hypoxia, Chemistry, hypoxia, Cancer, medicine.disease, 030104 developmental biology, 030220 oncology & carcinogenesis, Cancer cell |
| الوصف: | Extracellular vesicles (EVs) released from cancer cells contribute to various malignant phenotypes of cancer, including metastasis, cachexia, and angiogenesis. Although DNA, mRNAs, miRNAs, and proteins contained in EVs have been extensively studied, the function of metabolites in EVs remains unclear. In this study, we performed a comprehensive metabolomic analysis of pancreatic cancer cells, PANC-1, cultured under different oxygen concentrations, and small EVs (sEVs) released from them, considering the fact that hypoxia contributes to the malignant behavior of cells in pancreatic cancer, which is a poorly diagnosed cancer. sEVs were collected by ultracentrifugation, and hydrophilic metabolites were analyzed using capillary ion chromatography-mass spectrometry and liquid chromatography-mass spectrometry, and lipids were analyzed by supercritical fluid chromatography-tandem mass spectrometry. A total of 140 hydrophilic metabolites and 494 lipids were detected in sEVs, and their profiles were different from those in cells. In addition, the metabolomic profile of sEVs was observed to change under hypoxic stress, and an increase in metabolites involved in angiogenesis was also detected. We reveal the hallmark of the metabolites contained in sEVs and the effect of tumor hypoxia on their profiles, which may help in understanding EV-mediated cancer malignancy. |
| وصف الملف: | application/pdf |
| تدمد: | 2218-1989 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c46616d782a2a5ef7f2c7e6f45060d6 https://pubmed.ncbi.nlm.nih.gov/33915936 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2c46616d782a2a5ef7f2c7e6f45060d6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22181989 |
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