A novel long non-coding RNA in the rheumatoid arthritis risk locus TRAF1-C5 influences C5 mRNA levels
| العنوان: | A novel long non-coding RNA in the rheumatoid arthritis risk locus TRAF1-C5 influences C5 mRNA levels |
|---|---|
| المؤلفون: | T C Messemaker, M Frank-Bertoncelj, R B Marques, A Adriaans, A M Bakker, N Daha, S Gay, T W Huizinga, R E M Toes, H M M Mikkers, F Kurreeman |
| المساهمون: | University of Zurich, Kurreeman, F |
| المصدر: | Genes and Immunity, 17(2), 85-92 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | 0301 basic medicine, Untranslated region, 2716 Genetics (clinical), Genotype, Transcription, Genetic, Primary Cell Culture, Immunology, 610 Medicine & health, Locus (genetics), Biology, Quantitative trait locus, Polymorphism, Single Nucleotide, Monocytes, Arthritis, Rheumatoid, 03 medical and health sciences, 1311 Genetics, Cell Line, Tumor, Genetics, Humans, Genetic Predisposition to Disease, RNA, Messenger, RNA, Small Interfering, Gene, Genetics (clinical), Alpha-Amanitin, Nucleic Acid Synthesis Inhibitors, 2403 Immunology, Gene knockdown, Messenger RNA, Synovial Membrane, 10051 Rheumatology Clinic and Institute of Physical Medicine, RNA, Fibroblasts, Molecular biology, Long non-coding RNA, 030104 developmental biology, Genetic Loci, Hepatocytes, DNA, Intergenic, RNA, Long Noncoding |
| الوصف: | Long non-coding RNAs (lncRNAs) can regulate the transcript levels of genes in the same genomic region. These locally acting lncRNAs have been found deregulated in human disease and some have been shown to harbour quantitative trait loci (eQTLs) in autoimmune diseases. However, lncRNAs linked to the transcription of candidate risk genes in loci associated to rheumatoid arthritis (RA) have not yet been identified. The TRAF1 and C5 risk locus shows evidence of multiple eQTLs and transcription of intergenic non-coding sequences. Here, we identified a non-coding transcript (C5T1lncRNA) starting in the 3' untranslated region (UTR) of C5. RA-relevant cell types express C5T1lncRNA and RNA levels are further enhanced by specific immune stimuli. C5T1lncRNA is expressed predominantly in the nucleus and its expression correlates positively with C5 mRNA in various tissues (P=0.001) and in peripheral blood mononuclear cells (P=0.02) indicating transcriptional co-regulation. Knockdown results in a concurrent decrease in C5 mRNA levels but not of other neighbouring genes. Overall, our data show the identification of a novel lncRNA C5T1lncRNA that is fully located in the associated region and influences transcript levels of C5, a gene previously linked to RA pathogenesis. |
| تدمد: | 1476-5470 1466-4879 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c4bff01b3c140a07b384d183a2939ff https://doi.org/10.1038/gene.2015.54 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2c4bff01b3c140a07b384d183a2939ff |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14765470 14664879 |
|---|