A New Pipeline to Automatically Segment and Semi-Automatically Measure Bone Length on 3D Models Obtained by Computed Tomography
| العنوان: | A New Pipeline to Automatically Segment and Semi-Automatically Measure Bone Length on 3D Models Obtained by Computed Tomography |
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| المؤلفون: | Xinli Qu, Alberto Roselló-Díez, John Tan Nguyen, Michael Doube, Chee Ho H’ng, Olga Panagiotopoulou, Santiago Beltran Diaz, Michael John de Veer |
| المساهمون: | Rosello-Diez, Alberto [0000-0002-5550-9846], Apollo - University of Cambridge Repository |
| المصدر: | Frontiers in Cell and Developmental Biology, Vol 9 (2021) Frontiers in Cell and Developmental Biology |
| بيانات النشر: | Frontiers Media S.A., 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Computer science, QH301-705.5, Pipeline (computing), 3d model, Computed tomography, bone growth and development, Measure (mathematics), micro-CT (computed tomography), Cell and Developmental Biology, Length measurement, MIMICS, Methods, medicine, Biology (General), Measure (data warehouse), medicine.diagnostic_test, business.industry, Pattern recognition, Histology, Cell Biology, skeletal phenotype analysis, Thresholding, Bone length, Pipeline transport, BoneJ, limb evolution, Artificial intelligence, business, Ex vivo, Developmental Biology |
| الوصف: | The characterization of developmental phenotypes often relies on the accurate linear measurement of structures that are small and require laborious preparation. This is tedious and prone to errors, especially when repeated for the multiple replicates that are required for statistical analysis, or when multiple distinct structures have to be analysed. To address this issue, we have developed a pipeline for characterization of long-bone length and inter-vertebral distance using X-ray microtomography (XMT) scans. The pipeline involves semi-automated algorithms for automatic thresholding and fast interactive isolation and 3D-model generation of the main limb bones, using either the open-source ImageJ plugin BoneJ or the commercial Mimics Innovation Suite package. The tests showed the appropriate combination of scanning conditions and analysis parameters yields fast and comparable length results, highly correlated with the measurements obtained via ex vivo skeletal preparations. Moreover, since XMT is not destructive, the samples can be used afterwards for histology or other applications. Our new pipelines will help developmental biologists and evolutionary researchers to achieve fast, reproducible and non-destructive length measurement of bone samples from multiple animal species.Summary statementBeltran Diaz et al. present a semi-automated pipeline for fast and versatile characterization of bone length from micro-CT images of mouse developmental samples. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cad25ae3b2fb569f1f366967e0315ca https://www.frontiersin.org/articles/10.3389/fcell.2021.736574/full |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2cad25ae3b2fb569f1f366967e0315ca |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |