Physicochemical Characterization of Recombinant Human Nerve Growth Factor Produced in Insect Cells with a Baculovirus Vector
| العنوان: | Physicochemical Characterization of Recombinant Human Nerve Growth Factor Produced in Insect Cells with a Baculovirus Vector |
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| المؤلفون: | Jodi Fausnaugh, Natalie Saldou, Hardy Chan, Daryl K. Eggers, Eric Osen, Ken Straub, Jack Lile, Frank H. Collins, Joan Chow, Leo Gu, Raymond R. Townsend, Kurt Jarnagin, Hi-Shi Chaing, Binh T. Nguyen, Jim W. Barnett, Lisa Erdos |
| المصدر: | Journal of Neurochemistry. 57:1052-1061 |
| بيانات النشر: | Wiley, 1991. |
| سنة النشر: | 1991 |
| مصطلحات موضوعية: | Glycosylation, Insecta, Genetic Vectors, Molecular Sequence Data, Enzyme-Linked Immunosorbent Assay, Biology, Biochemistry, Cell Line, law.invention, Cellular and Molecular Neuroscience, chemistry.chemical_compound, law, Native state, Animals, Humans, Amino Acid Sequence, Isoelectric Point, Nerve Growth Factors, Peptide sequence, Chromatography, High Pressure Liquid, chemistry.chemical_classification, Isoelectric focusing, Recombinant Proteins, Amino acid, Molecular Weight, Isoelectric point, chemistry, Cell culture, Recombinant DNA, Baculoviridae, Plasmids |
| الوصف: | Recombinant human nerve growth factor (rhNGF) secreted by insect cells was purified by ion-exchange and reversed-phase chromatography to near homogeneity. The N-terminus of the secreted molecule was analogous to that of mouse salivary gland NGF. In its native conformation, the insect cell produced rhNGF molecules were homodimers consisting of 120 amino acid polypeptide chains. Mature rhNGF was found not to be significantly glycosylated (less than 0.08 mol of N-acetylglucosamine/mol of protein). The rhNGF was homogeneous with regard to molecular weight and amino acid sequence. Isoelectric focusing resolved the rhNGF into one major and one minor component. Because rhNGF from insect cells can be obtained in large quantities, purified to near homogeneity, and is similar to natural NGF with regard to physicochemical properties and biological activity, it is suitable for further evaluation in animal models as a therapeutic molecule for neurodegenerative diseases such as Alzheimer's disease. |
| تدمد: | 1471-4159 0022-3042 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ce9988524eb6a1dd21ac2d07e6e4093 https://doi.org/10.1111/j.1471-4159.1991.tb08256.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....2ce9988524eb6a1dd21ac2d07e6e4093 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14714159 00223042 |
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