PI3K /Akt/ CncC signaling pathway mediates the response to EPN‐Bt infection in Holotrichia parallela larvae
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| المؤلفون: | Er‐Tao Li, Han‐Jia Wu, Zhi‐Min Wang, Ke‐Bin Li, Shuai Zhang, Ya‐Zhong Cao, Jiao Yin |
| المصدر: | Pest Management Science. 79:1660-1673 |
| بيانات النشر: | Wiley, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Insect Science, General Medicine, Agronomy and Crop Science |
| الوصف: | Combining the entomopathogenic nematode (EPN), Heterorhabditis beicherriana LF strain and Bacillus thuringiensis (Bt) HBF-18 strain is a practical strategy to manage the larvae of Holotrichia parallela Motschulsky (white grubs). However, the mechanisms underlying the larval defense response to this combined biocontrol strategy are unknown.Herein, the activities of some antioxidant enzymes (SOD, POD, CAT) and some detoxifying enzymes (AChE, P-450, CarE, GST) in grubs showed an activation-inhibition trend throughout the EPN-Bt exposure time course. Eight potentially key antioxidant and detoxifying enzyme genes in response to EPN-Bt infection were identified from the midgut of grubs through RNA-seq. After silencing CAT, CarE18, and GSTs1, the enzyme activities were significantly decreased by 30.29%, 68.80%, and 34.63%, respectively. Meanwhile, the mortality of grubs was increased by 18.40%, 46.30%, and 42.59% after exposure to EPN-Bt for 1 day. Interestingly, the PI3K/Akt signaling pathway was significantly enriched in KEGG enrichment analysis, and the expression levels of phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), cap 'n' collar isoform-C (CncC), kelch-like ECH-associated protein 1 (Keap1), and CarE18 were all upregulated when exposed to EPN-Bt for 1 day. Furthermore, RNAi-mediated PI3K silencing showed a similar downregulated trend between PI3K/Akt/CncC and CarE18. Moreover, silencing PI3K rendered grubs more susceptible to EPN-Bt and accelerated symbiotic bacteria multiplication in grubs.These results suggest that PI3K/Akt/CncC pathway mediates the expression of CarE18 and participates in the defense response of H. parallela larvae against EPN-Bt infection. Our data provide valuable insights into the design of appropriate management strategies for this famous agricultural pest. |
| تدمد: | 1526-4998 1526-498X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2cf364f392b6bc3b4b16363a6b25a467 https://doi.org/10.1002/ps.7337 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi.dedup.....2cf364f392b6bc3b4b16363a6b25a467 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15264998 1526498X |
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