Control of gene expression during T cell activation: alternate regulation of mRNA transcription and mRNA stability
| العنوان: | Control of gene expression during T cell activation: alternate regulation of mRNA transcription and mRNA stability |
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| المؤلفون: | Myriam Gorospe, Jill M. Ray, Chris Cheadle, Jinshui Fan, Kevin G. Becker, Yoon S. Cho-Chung, Lana Do, Thomas Werner |
| المصدر: | BMC Genomics BMC Genomics, Vol 6, Iss 1, p 75 (2005) |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Nuclear gene, Time Factors, lcsh:QH426-470, Transcription, Genetic, lcsh:Biotechnology, T-Lymphocytes, Biology, Lymphocyte Activation, Polymerase Chain Reaction, Jurkat Cells, Transcription (biology), lcsh:TP248.13-248.65, Gene expression, P-bodies, Genetics, Cluster Analysis, Humans, RNA, Messenger, Gene, Oligonucleotide Array Sequence Analysis, Regulation of gene expression, Cell Nucleus, Gene knockdown, Gene Expression Profiling, Ionomycin, NF-kappa B, Computational Biology, Genomics, Molecular biology, Gene expression profiling, lcsh:Genetics, Gene Expression Regulation, Dactinomycin, Tetradecanoylphorbol Acetate, Poly A, Biotechnology, Research Article |
| الوصف: | Background Microarray technology has become highly valuable for identifying complex global changes in gene expression patterns. The effective correlation of observed changes in gene expression with shared transcription regulatory elements remains difficult to demonstrate convincingly. One reason for this difficulty may result from the intricate convergence of both transcriptional and mRNA turnover events which, together, directly influence steady-state mRNA levels. Results In order to investigate the relative contribution of gene transcription and changes in mRNA stability regulation to standard analyses of gene expression, we used two distinct microarray methods which individually measure nuclear gene transcription and changes in polyA mRNA gene expression. Gene expression profiles were obtained from both polyA mRNA (whole-cell) and nuclear run-on (newly transcribed) RNA across a time course of one hour following the activation of human Jurkat T cells with PMA plus ionomycin. Comparative analysis revealed that regulation of mRNA stability may account for as much as 50% of all measurements of changes in polyA mRNA in this system, as inferred by the absence of any corresponding regulation of nuclear gene transcription activity for these groups of genes. Genes which displayed dramatic elevations in both mRNA and nuclear run-on RNA were shown to be inhibited by Actinomycin D (ActD) pre-treatment of cells while large numbers of genes regulated only through altered mRNA turnover (both up and down) were ActD-resistant. Consistent patterns across the time course were observed for both transcribed and stability-regulated genes. Conclusion We propose that regulation of mRNA stability contributes significantly to the observed changes in gene expression in response to external stimuli, as measured by high throughput systems. |
| تدمد: | 1471-2164 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d053f6106016ca2f61b39effdd6b7bc https://pubmed.ncbi.nlm.nih.gov/15907206 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2d053f6106016ca2f61b39effdd6b7bc |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14712164 |
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