Induced pluripotent stem cells derived from human amnion in chemically defined conditions
| العنوان: | Induced pluripotent stem cells derived from human amnion in chemically defined conditions |
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| المؤلفون: | Simon P. Hoerstrup, Benedikt Weber, Jaroslav Slamecka, Laurie B. Owen, Steven McClellan, Javier A. Laurini, Elizabeth A. Manci, Anna Wilk |
| المساهمون: | University of Zurich, Slamecka, Jaroslav |
| المصدر: | Cell Cycle. 17:330-347 |
| بيانات النشر: | Informa UK Limited, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Transcription, Genetic, Induced Pluripotent Stem Cells, 610 Medicine & health, Germ layer, Biology, 1309 Developmental Biology, 1307 Cell Biology, 03 medical and health sciences, Report, 1312 Molecular Biology, Humans, Gene Regulatory Networks, Amnion, Induced pluripotent stem cell, Fetal Stem Cells, Cell Shape, Molecular Biology, Cells, Cultured, Mesenchymal stem cell, Teratoma, Mesenchymal Stem Cells, 11359 Institute for Regenerative Medicine (IREM), Cell Biology, Embryonic stem cell, Cell biology, 030104 developmental biology, Reprogramming, Developmental biology, Biomarkers, Developmental Biology, Adult stem cell |
| الوصف: | Fetal stem cells are a unique type of adult stem cells that have been suggested to be broadly multipotent with some features of pluripotency. Their clinical potential has been documented but their upgrade to full pluripotency could open up a wide range of cell-based therapies particularly suited for pediatric tissue engineering, longitudinal studies or disease modeling. Here we describe episomal reprogramming of mesenchymal stem cells from the human amnion to pluripotency (AM-iPSC) in chemically defined conditions. The AM-iPSC expressed markers of embryonic stem cells, readily formed teratomas with tissues of all three germ layers present and had a normal karyotype after around 40 passages in culture. We employed novel computational methods to determine the degree of pluripotency from microarray and RNA sequencing data in these novel lines alongside an iPSC and ESC control and found that all lines were deemed pluripotent, however, with variable scores. Differential expression analysis then identified several groups of genes that potentially regulate this variability in lines within the boundaries of pluripotency, including metallothionein proteins. By further studying this variability, characteristics relevant to cell-based therapies, like differentiation propensity, could be uncovered and predicted in the pluripotent stage. |
| تدمد: | 1551-4005 1538-4101 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d1ea1c1c5efe87e85444d498ec2428c https://doi.org/10.1080/15384101.2017.1403690 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2d1ea1c1c5efe87e85444d498ec2428c |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 15514005 15384101 |
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