Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus
| العنوان: | Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus |
|---|---|
| المؤلفون: | Dimos Poulikakos, Alexander A. Mironov, Paulina Nastały, Galina V. Beznoussenko, Divya Purushothaman, Tobias Lendenmann, Orso Maria Romano, Aldo Ferrari, Marco Cosentino Lagomarsino, Stefano Marchesi, Gururaj Rao Kidiyoor, Stefania Lavore, Paolo Maiuri, Alessandro Poli |
| المساهمون: | Nastaly, P, Purushothaman, D, Marchesi, S, Poli, A, Lendenmann, T, Kidiyoor, Gr, Beznoussenko, Gv, Lavore, S, Romano, Om, Poulikakos, D, Lagomarsino, Mc, Mironov, Aa, Ferrari, A, Maiuri, P |
| المصدر: | Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020) Nature Communications, 11 (1) Nature Communications |
| بيانات النشر: | ETH Zurich, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Nuclear Envelope, Polarity (physics), Science, Blotting, Western, Emerin, Fluorescent Antibody Technique, General Physics and Astronomy, Article, General Biochemistry, Genetics and Molecular Biology, Cell Line, Myoblasts, 03 medical and health sciences, 0302 clinical medicine, Microscopy, Electron, Transmission, Cell polarity, medicine, Humans, Cell migration, Nuclear membrane, Nuclear protein, Cytoskeleton, lcsh:Science, Cell Nucleus, Microscopy, Confocal, Multidisciplinary, Chemistry, Endoplasmic reticulum, digestive, oral, and skin physiology, Membrane Proteins, Nuclear Proteins, General Chemistry, Cell biology, 030104 developmental biology, medicine.anatomical_structure, RNA Interference, lcsh:Q, Nucleus, 030217 neurology & neurosurgery |
| الوصف: | Cell polarity refers to the intrinsic asymmetry of cells, including the orientation of the cytoskeleton. It affects cell shape and structure as well as the distribution of proteins and organelles. In migratory cells, front-rear polarity is essential and dictates movement direction. While the link between the cytoskeleton and nucleus is well-studied, we aim to investigate if front-rear polarity can be transmitted to the nucleus. We show that the knock-down of emerin, an integral protein of the nuclear envelope, abolishes preferential localization of several nuclear proteins. We propose that the frontally biased localization of the endoplasmic reticulum, through which emerin reaches the nuclear envelope, is sufficient to generate its observed bias. In primary emerin-deficient myoblasts, its expression partially rescues the polarity of the nucleus. Our results demonstrate that front-rear cell polarity is transmitted to the nucleus and that emerin is an important determinant of nuclear polarity. Nature Communications, 11 (1) ISSN:2041-1723 |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| تدمد: | 2041-1723 |
| DOI: | 10.3929/ethz-b-000414003 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d35151867fe2a7ed13fa605497b5d94 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2d35151867fe2a7ed13fa605497b5d94 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20411723 |
|---|---|
| DOI: | 10.3929/ethz-b-000414003 |