A Widely Distributed Biosynthetic Cassette Is Responsible for Diverse Plant Side Chain Cross‐Linked Cyclopeptides**
العنوان: | A Widely Distributed Biosynthetic Cassette Is Responsible for Diverse Plant Side Chain Cross‐Linked Cyclopeptides** |
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المؤلفون: | Stella T. Lima, Brigitte G. Ampolini, Ethan B. Underwood, Tyler N. Graf, Cody E. Earp, Imani C. Khedi, Michael A. Pasquale, Jonathan R. Chekan |
المصدر: | Angewandte Chemie. 135 |
بيانات النشر: | Wiley, 2023. |
سنة النشر: | 2023 |
مصطلحات موضوعية: | General Chemistry, General Medicine, Catalysis |
الوصف: | Cyclopeptide alkaloids are an abundant class of plant cyclopeptides with over 200 analogs described and bioactivities ranging from analgesic to antiviral. While these natural products have been known for decades, their biosynthetic basis remains unclear. Using a transcriptome-mining approach, we link the cyclopeptide alkaloids from Ceanothus americanus to dedicated RiPP precursor peptides and identify new, widely distributed split BURP peptide cyclase containing gene clusters. Guided by our bioinformatic analysis, we identify and isolate new cyclopeptides from Coffea arabica, which we named arabipeptins. Reconstitution of the enzyme activity for the BURP found in the biosynthesis of arabipeptin A validates the activity of the newly discovered split BURP peptide cyclases. These results expand our understanding of the biosynthetic pathways responsible for diverse cyclic plant peptides and suggest that these side chain cross-link modifications are widely distributed in eudicots. |
تدمد: | 1521-3757 0044-8249 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e35fd221ce9ed069f438ae6c3588fe1 https://doi.org/10.1002/ange.202218082 |
حقوق: | OPEN |
رقم الأكسشن: | edsair.doi.dedup.....2e35fd221ce9ed069f438ae6c3588fe1 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 15213757 00448249 |
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