Simultaneous Detection and HER2 Profiling of Circulating Breast Cancer Cells in Clinical Patients Using a Rare Cell Sorter
| العنوان: | Simultaneous Detection and HER2 Profiling of Circulating Breast Cancer Cells in Clinical Patients Using a Rare Cell Sorter |
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| المؤلفون: | Tomoko, Mori, Atsuko, Furukawa, Turan, Bilal, Aya, Ueda, Hiroko, Bando, Taisuke, Masuda, Fumihito, Arai, Satoshi, Matsusaka |
| المصدر: | Anticancer Research. 42:4879-4886 |
| بيانات النشر: | Anticancer Research USA Inc., 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Cancer Research, Oncology, Receptor, ErbB-2, Biomarkers, Tumor, Humans, Breast Neoplasms, Cell Count, Female, General Medicine, Flow Cytometry, Neoplastic Cells, Circulating |
| الوصف: | This study describes a rare cell sorter (RCS) method to detect circulating tumor cells (CTCs) and CTC clusters in whole blood without pretreatment.We collected samples from breast cancer patients at the University of Tsukuba Hospital. A total of 15 whole-blood specimens from patients with breast cancer were collected and analyzed via a microfluidics chip, fluorescence-conjugated antibody staining, and fluorescence microscopy. Of 15 total cases, eight were analyzed by RCS ver3 and seven were analyzed by RCS ver3.5 to reveal potential clinical differences in scanning methods. We then examined the HER2 status on 4 of the 15 patients using our RCS system.RCS efficiently detected all subtypes of CTCs and CTC clusters from the peripheral blood of cancer patients. The concordance rate of HER2 status between tissue and CTCs in 4 tested clinical samples was 100%.RCS is a non-invasive method that allows for simultaneous detection of CTCs, cluster presence, and surface marker (e.g., HER2) status. Frequent sampling is, thus, possible and the large amount of data obtained will be clinically useful to predict response to therapy as well as plan adjunct support therapies in cancer patients. |
| تدمد: | 1791-7530 0250-7005 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e66a1640f8f6ee10a8b24c0ec1e2548 https://doi.org/10.21873/anticanres.15993 |
| رقم الأكسشن: | edsair.doi.dedup.....2e66a1640f8f6ee10a8b24c0ec1e2548 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17917530 02507005 |
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