Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array
| العنوان: | Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array |
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| المؤلفون: | Gero Steinberg, Sreedhar Kilaru, Martin Schuster, Jérôme Collemare, Yvonne Roger, Gero Fink |
| المصدر: | Molecular Biology of the Cell, 22(19), 3645-3657 Molecular Biology of the Cell Molecular Biology of the Cell 22 (2011) 19 |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Cytoplasm, Dynein, Hyphae, Motility, Kinesins, Endosomes, macromolecular substances, Biology, Microtubules, molecular motors, vesicle transport, 03 medical and health sciences, 0302 clinical medicine, polarity orientation, Microtubule, Tubulin, Cell polarity, Growing cell tip, Molecular motor, Ustilago, intracellular-transport, lipid-droplet transport, tug-of-war, cargo transport, Molecular Biology, 030304 developmental biology, 0303 health sciences, Cell Polarity, Dyneins, Microtubule organizing center, fungus ustilago-maydis, Cell Biology, Articles, cytoplasmic dynein, Cell biology, Laboratorium voor Phytopathologie, caenorhabditis-elegans, Membrane Trafficking, Laboratory of Phytopathology, Kinesin, EPS, 030217 neurology & neurosurgery, Microtubule-Organizing Center |
| الوصف: | We studied molecular motors in long-range motility of early endosomes (EEs) in a fungal model system that contains a bipolar, dendrite-like microtubule (MT) array. Dynein moves retrograde EEs over ∼10 μm, before kinesin-3 takes over for a further ∼80 μm along antipolar MT bundles. Thus kinesin-3 is the major motor for retrograde EE motility. The polarity of microtubules (MTs) determines the motors for intracellular motility, with kinesins moving to plus ends and dynein to minus ends. In elongated cells of Ustilago maydis, dynein is thought to move early endosomes (EEs) toward the septum (retrograde), whereas kinesin-3 transports them to the growing cell tip (anterograde). Occasionally, EEs run up to 90 μm in one direction. The underlying MT array consists of unipolar MTs at both cell ends and antipolar bundles in the middle region of the cell. Cytoplasmic MT-organizing centers, labeled with a γ-tubulin ring complex protein, are distributed along the antipolar MTs but are absent from the unipolar regions. Dynein colocalizes with EEs for 10–20 μm after they have left the cell tip. Inactivation of temperature-sensitive dynein abolishes EE motility within the unipolar MT array, whereas long-range motility is not impaired. In contrast, kinesin-3 is continuously present, and its inactivation stops long-range EE motility. This indicates that both motors participate in EE motility, with dynein transporting the organelles through the unipolar MT array near the cell ends, and kinesin-3 taking over at the beginning of the medial antipolar MT array. The cooperation of both motors mediates EE movements over the length of the entire cell. |
| وصف الملف: | application/octet-stream; application/pdf |
| اللغة: | English |
| تدمد: | 1059-1524 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e72d9dfd06c7a541ec2c375dd70f006 https://doi.org/10.1091/mbc.e11-03-0217 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2e72d9dfd06c7a541ec2c375dd70f006 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10591524 |
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