Large plasmids encoding antibiotic resistance and localized-like adherence in atypical enteropathogenic Escherichia coli strains
| العنوان: | Large plasmids encoding antibiotic resistance and localized-like adherence in atypical enteropathogenic Escherichia coli strains |
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| المؤلفون: | Mariane Vedovatti Monfardini, Scarlat S. Silva, Isabel C. A. Scaletsky |
| المصدر: | BMC Microbiology, Vol 20, Iss 1, Pp 1-8 (2020) BMC Microbiology |
| بيانات النشر: | BMC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Microbiology (medical), Transposable element, Gene Transfer, Horizontal, Sequence analysis, Tetracycline, Mutant, lcsh:QR1-502, Microbial Sensitivity Tests, Biology, Antimicrobial resistance, Microbiology, Bacterial Adhesion, lcsh:Microbiology, Localized adherence-like, Enteropathogenic Escherichia coli, 03 medical and health sciences, 0302 clinical medicine, Plasmid, Kanamycin, Drug Resistance, Bacterial, medicine, Humans, 030212 general & internal medicine, Gene, 0303 health sciences, 030306 microbiology, Escherichia coli Proteins, HEK 293 cells, Atypical enteropathogenic Escherichia coli, Anti-Bacterial Agents, Mutagenesis, Insertional, Conjugation, Genetic, Ampicillin, Fimbriae Proteins, Research Article, HeLa Cells, medicine.drug, Plasmids |
| الوصف: | Background In previous studies, we have shown that atypical enteropathogenic Escherichia coli (aEPEC) strains are important diarrheal pathogens among Brazilian children. In the characterization of a collection of 126 aEPEC strains, we identified 29 strains expressing the localized-like adherence (LAL) pattern on HEp-2 cells and harboring large plasmids in the range of 60 to 98 MDa. In this study, we examined 18 of these strains for their ability to transfer the LAL phenotype to a E. coli K-12 C600 strain. Results In conjugation experiments, using eight strains which were resistant to one or more antimicrobials and positive for F-pili genes (traA), we were able to cotransfer antimicrobial resistance markers along with adhesion genes. By transforming E. coli DH5α with plasmid DNA from strains A46 (pIS46), A66 (pIS66) and A102 (pIS102), we were able to demonstrate that genes encoding ampicillin, tetracycline and LAL were encoded on a 98-MDa conjugative plasmid. To identify a gene responsible for LAL, we constructed a transposon mutant library of A102 strain. Among 18 mutants that did not adhere to HeLa cells, four carried insertions within fimbrial genes (fimA and traJ) and agglutinin genes (tia and hek). Using these Tn5 mutants as donors, we were able to obtain kanamycin-resistant E. coli MA3456 transconjugants. Sequence analysis of the plasmid genes revealed a region exhibit to 80 and 73% amino acid similarities to the agglutinins Tia and Hek, respectively. Conclusion In this study, we have identified three large conjugative plasmids, pIS46, pIS66 and pIS102, coding for antimicrobial resistance and localized-like adherence (LAL) to HeLa cells. In addition, we identified a tia/hek homolog encoded on the pIS102 plasmid, which seems to be involved in adhesion of A102 strain. |
| اللغة: | English |
| تدمد: | 1471-2180 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ecd1172b5ad893b4eff7de62376592f http://link.springer.com/article/10.1186/s12866-020-01809-4 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2ecd1172b5ad893b4eff7de62376592f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712180 |
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