Knockdown of Rhotekin 2 expression suppresses proliferation and induces apoptosis in colon cancer cells
| العنوان: | Knockdown of Rhotekin 2 expression suppresses proliferation and induces apoptosis in colon cancer cells |
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| المؤلفون: | Xue-Qin Pang, Chen Ma, Rui Li, Weichang Chen, Chuanyong Mu, Xudong Pan, Dongtao Shi, Guangbo Zhang |
| المصدر: | Oncology Letters |
| بيانات النشر: | Spandidos Publications, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Colorectal cancer, Biology, medicine.disease_cause, 03 medical and health sciences, 0302 clinical medicine, medicine, Gene silencing, rhotekin 2, Oncogene, apoptosis, Wnt signaling pathway, Cancer, Articles, Cell cycle, medicine.disease, Molecular medicine, Molecular biology, digestive system diseases, cell proliferation, 030104 developmental biology, Oncology, 030220 oncology & carcinogenesis, cell cycle, colon cancer cells, Carcinogenesis |
| الوصف: | Colon cancer is one of the most common malignant tumors in the human body, ranking second as a gastrointestinal tumor. It has a high incidence in Europe, America and China and more than 1 million new cases of colon cancer are reported worldwide each year. The incidence of colon cancer in China has increased from 12/0.1 million in the early 1970s to 56/0.1 million at present with an annual growth rate of 4.2%, which far exceeds the international level (2%). Rhotekin (RTKN) 2, a Rho-guanosine triphosphatase (GTPase) effector, has been reported to be anti-apoptotic. However, the molecular mechanism underlying the biological function of RTKN2 in colon cancer remains unknown. The present study investigated whether the mRNA expression level of RTKN2 was markedly higher in 30 human colon cancer specimens compared with adjacent non-cancerous tissues. The results showed that the protein expression level of RTKN2 was significantly higher in SW480 and HCT116 cells, compared with HIEC cells. Knockdown of RTKN2 in the SW480 and HCT116 colon cancer cells, by lentivirus-mediated RNA interference led to the notable inhibition of cell proliferation and cell cycle progression, by reducing the expression levels of the PCDA, Cyclin D1 and c-myc cell cycle-associated proteins. The inhibitory effect of RTKN2 silencing on the proliferation of colon cancer cells may be partially realized by inhibiting the Wnt/β-catenin signaling pathway. Furthermore, the silencing of RTKN2 in the cells induced apoptosis by reducing the expression level of Bax and increasing the expression level of Bcl2. These results show that RTKN2 is involved in the carcinogenesis and progression of human colon cancer, indicating that RTKN2 may be a molecular target in colon cancer therapy. |
| تدمد: | 1792-1082 1792-1074 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ed0e850d2eb5d8a36b41b13aaaf505c https://doi.org/10.3892/ol.2017.7182 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2ed0e850d2eb5d8a36b41b13aaaf505c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
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