Comparison of three different methods to detect bone marrow involvement in patients with neuroblastoma
| العنوان: | Comparison of three different methods to detect bone marrow involvement in patients with neuroblastoma |
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| المؤلفون: | Peter F. Ambros, Karoline Ehlert, Felix Schriegel, Marie Bernkopf, Holger N. Lode, Nikolai Siebert, Inge M. Ambros, Sabine Taschner-Mandl, Guenter Henze, Uwe Grunwald |
| المصدر: | Journal of Cancer Research and Clinical Oncology. 148:2581-2588 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Cancer Research, medicine.medical_specialty, Immunofluorescence, Flow cytometry, Neuroblastoma, Bone Marrow, Internal medicine, medicine, Humans, Prospective Studies, Child, Prospective cohort study, In Situ Hybridization, Fluorescence, Retrospective Studies, Hematology, medicine.diagnostic_test, business.industry, General Medicine, medicine.disease, Minimal residual disease, medicine.anatomical_structure, Oncology, Bone marrow, business, Nuclear medicine, Fluorescence in situ hybridization |
| الوصف: | Purpose Neuroblastoma (NB) is the most frequent extracranial tumor in children. The detection of bone marrow (BM) involvement is crucial for correct staging and risk-adapted treatment. We compared three methods regarding the detection of NB involvement in BM. Methods Eighty-one patients with NB were included in this retrospective study. BM samples were obtained at designated time points at study entry and during treatment or follow-up. The diagnostic tools for BM analysis included cytomorphology (CM), flow cytometry (FCM) and automatic immunofluorescence plus fluorescence in situ hybridization (AIPF). Results We analyzed 369 aspirates in 81 patients in whom AIPF, CM, and FCM were simultaneously available. During the observation period, NB cells were detected in 86/369 (23.3%) cases, by CM in 32/369 (8.7%), by FCM in 52 (14.1%), and by AIPF in 72 (19.5%) samples. AIPF and/or FCM confirmed all positive results obtained in CM and detected 11 additional positive BM aspirates in 294 CM negative samples (p Conclusion The combination of AIPF/FCM yielded the highest detection rate of NB cells in BM. AIPF was the single, most sensitive method in detecting these cells. Although CM did not provide any additional positive results, it is still a useful, readily available and cost-effective tool. The prognostic significance of FCM and AIPF should be confirmed in a prospective study with a larger number of patients. |
| تدمد: | 1432-1335 0171-5216 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f18ac63e55c05d24e480664eb1a1359 https://doi.org/10.1007/s00432-021-03780-7 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2f18ac63e55c05d24e480664eb1a1359 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14321335 01715216 |
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