Infiltration of T Cells into a Three-Dimensional Psoriatic Skin Model Mimics Pathological Key Features
| العنوان: | Infiltration of T Cells into a Three-Dimensional Psoriatic Skin Model Mimics Pathological Key Features |
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| المؤلفون: | Mélissa Simard, Sophie Morin, Isabelle Lorthois, Roxane Pouliot |
| المصدر: | International Journal of Molecular Sciences Volume 20 Issue 7 International Journal of Molecular Sciences, Vol 20, Iss 7, p 1670 (2019) |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Keratinocytes, Male, 0301 basic medicine, 3D model, Pathology, T-Lymphocytes, Lymphocyte Activation, Epithelium, lcsh:Chemistry, 0302 clinical medicine, Cell Movement, Medicine, lcsh:QH301-705.5, Spectroscopy, Skin, integumentary system, Cell Differentiation, Dermis, General Medicine, psoriasis, adaptive immunity, Middle Aged, Acquired immune system, Phenotype, 3. Good health, Computer Science Applications, 030220 oncology & carcinogenesis, tissue engineering, Female, Inflammation Mediators, medicine.symptom, Immunocompetence, Infiltration (medical), Signal Transduction, medicine.medical_specialty, T cells, Inflammation, Models, Biological, Article, Catalysis, Proinflammatory cytokine, Inorganic Chemistry, 03 medical and health sciences, Psoriasis, Humans, Physical and Theoretical Chemistry, Molecular Biology, Cell Proliferation, business.industry, Organic Chemistry, medicine.disease, In vitro, Methotrexate, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, inflammation, business, Epidermal thickening |
| الوصف: | Psoriasis is an autoimmune chronic dermatosis that is T cell-mediated, characterized by epidermal thickening, aberrant epidermal differentiation and inflammatory infiltrates, with a dominant Th1 and Th17 profile. Additional in vitro models are required to study the complex interactions between activated T cells and skin cells, and to develop new, more effective treatments. We have therefore sought to model this psoriatic inflammation by the generation of tissue-engineered immunocompetent tissues, and we have investigated the response of activated T-cell infiltration in models produced with lesional psoriatic skin cells on major hallmarks of psoriasis. The immunocompetent lesional skin model displayed a delayed onset of epidermal differentiation, an hyperproliferation of the basal keratinocytes, a drastic increase in the secretion of proinflammatory cytokines, and a disturbed expression of key transcription factors, as observed in lesional plaques, suggesting a crucial importance of combining the pathological phenotype of cutaneous cells to T cells in order to generate a relevant model for psoriasis. Finally, we found this skin model to be responsive to methotrexate treatment, making it a valuable tool for drug development. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1422-0067 |
| DOI: | 10.3390/ijms20071670 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f53e5b3fed2bbb6aa7de8638184e197 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2f53e5b3fed2bbb6aa7de8638184e197 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14220067 |
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| DOI: | 10.3390/ijms20071670 |