Is adipose tissue suitable for detection of (synthetic) cannabinoids? A comparative study analyzing antemortem and postmortem specimens following pulmonary administration of JWH-210, RCS-4, as well as ∆9-tetrahydrocannabinol to pigs
| العنوان: | Is adipose tissue suitable for detection of (synthetic) cannabinoids? A comparative study analyzing antemortem and postmortem specimens following pulmonary administration of JWH-210, RCS-4, as well as ∆9-tetrahydrocannabinol to pigs |
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| المؤلفون: | Peter H. Schmidt, Christina Körbel, Nadine Schaefer, Hans H. Maurer, Markus R. Meyer, Matthias W. Laschke, Frederike Nordmeier, Ann-Katrin Kröll, Michael D. Menger |
| المصدر: | Archives of Toxicology |
| بيانات النشر: | Springer Berlin Heidelberg, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Chronic exposure, Male, medicine.medical_specialty, Indoles, Time Factors, Synthetic cannabinoids, Swine, Health, Toxicology and Mutagenesis, Metabolite, Adipose tissue, Naphthalenes, Toxicology, Body weight, 01 natural sciences, Specimen Handling, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Tandem Mass Spectrometry, Internal medicine, medicine, Pulmonary administration, Animals, 030216 legal & forensic medicine, Dronabinol, Tetrahydrocannabinol, Lung, Cannabinoids, 010401 analytical chemistry, General Medicine, RCS-4, 0104 chemical sciences, Endocrinology, Respiratory Tract Absorption, chemistry, Adipose Tissue, JWH-210, Pigs, Adipose tissue, postmortem redistribution, medicine.drug, Toxicokinetics and Metabolism, Chromatography, Liquid |
| الوصف: | Examining fatal poisonings, chronic exposure may be reflected by the concentration in tissues known for long-term storage of drugs. Δ9-tetrahydrocannabinol (THC) persists in adipose tissue (AT), but sparse data on synthetic cannabinoids (SC) are available. Thus, a controlled pig study evaluating antemortem (AM) disposition and postmortem (PM) concentration changes of the SC 4-ethylnaphthalene-1-yl-(1-pentylindole-3-yl)methanone (JWH-210) and 2-(4-methoxyphenyl)-1-(1-pentyl-indole-3-yl)methanone (RCS-4) as well as THC in AT was performed. The drugs were administered pulmonarily (200 µg/kg body weight) to twelve pigs. Subcutaneous (s.c.) AT specimens were collected after 15 and 30 min and then hourly up to 8 h. At the end, pigs were sacrificed and s.c., perirenal, and dorsal AT specimens were collected. The carcasses were stored at room temperature (RT; n = 6) or 4 °C (n = 6) and specimens were collected after 24, 48, and 72 h. After homogenization in acetonitrile and standard addition, LC–MS/MS was performed. Maximum concentrations were reached 0.5–2 h after administration amounting to 21 ± 13 ng/g (JWH-210), 24 ± 13 ng/g (RCS-4), and 22 ± 20 ng/g (THC) and stayed at a plateau level. Regarding the metabolites, very low concentrations of N-hydroxypentyl-RCS-4 (HO-RCS-4) were detected from 0.5 to 8 h. PM concentrations of parent compounds did not change significantly (p > 0.05) over time under both storage conditions. Concentrations of HO-RCS-4 significantly (p |
| اللغة: | English |
| تدمد: | 1432-0738 0340-5761 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f5783445df218442ecd2fc618d1ef87 http://europepmc.org/articles/PMC7502066 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi.dedup.....2f5783445df218442ecd2fc618d1ef87 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14320738 03405761 |
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