Abundance of two human preadipocyte subtypes with distinct capacities for replication, adipogenesis, and apoptosis varies among fat depots
| العنوان: | Abundance of two human preadipocyte subtypes with distinct capacities for replication, adipogenesis, and apoptosis varies among fat depots |
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| المؤلفون: | Ada Koo, Tamara Tchkonia, James L. Kirkland, Tamar Pirtskhalava, Nino Giorgadze, Yourka D. Tchoukalova, Dharmaraj Chinnappan, Iordanes Karagiannides, Michael D. Jensen, Andrew Cartwright, R. Armour Forse, Michael Stevenson |
| المصدر: | American Journal of Physiology-Endocrinology and Metabolism. 288:E267-E277 |
| بيانات النشر: | American Physiological Society, 2005. |
| سنة النشر: | 2005 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Tumor Necrosis Factor-alpha, Physiology, Stem Cells, Endocrinology, Diabetes and Metabolism, Adipose tissue, Apoptosis, Cell Communication, Middle Aged, Biology, Endocrinology, Adipose Tissue, Adipogenesis, Physiology (medical), Internal medicine, Adipocytes, CCAAT-Enhancer-Binding Protein-alpha, medicine, Humans, Female, Cell Division, Cells, Cultured |
| الوصف: | Fat depots vary in function and size. The preadipocytes that fat cells develop from exhibit distinct regional characteristics that persist in culture. Human abdominal subcutaneous cultured preadipocytes undergo more extensive lipid accumulation, higher adipogenic transcription factor expression, and less TNF-α-induced apoptosis than omental preadipocytes. We found higher replicative potential in subcutaneous and mesenteric than in omental preadipocytes. In studies of colonies arising from single preadipocytes, two preadipocyte subtypes were found, one capable of more extensive replication, differentiation, and adipogenic transcription factor expression and less apoptosis in response to TNF-α than the other. The former was more abundant in subcutaneous and mesenteric than in omental preadipocyte populations, potentially contributing to regional variation in replication, differentiation, and apoptosis. Both subtypes were found in strains derived from single human preadipocytes stably expressing telomerase, confirming that both subtypes are of preadipocyte lineage. After subcloning of cells of either subtype, both subtypes were found, indicating that switching can occur between subtypes. Thus proportions of preadipocyte subtypes with distinct cell-dynamic properties vary among depots, potentially permitting tissue plasticity through subtype selection during development. Furthermore, mesenteric preadipocyte cell-dynamic characteristics are distinct from omental cells, indicating that visceral fat depots are not functionally uniform. |
| تدمد: | 1522-1555 0193-1849 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fc33112a4ddea0be436cf9b220d766d https://doi.org/10.1152/ajpendo.00265.2004 |
| رقم الأكسشن: | edsair.doi.dedup.....2fc33112a4ddea0be436cf9b220d766d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15221555 01931849 |
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